CHARON PHAGES - SAFER DERIVATIVES OF BACTERIOPHAGE-LAMBDA FOR DNA CLONING
CHARON PHAGES - SAFER DERIVATIVES OF BACTERIOPHAGE-LAMBDA FOR DNA CLONING
复制标题
DOI：
10.1126/science.847462
复制
发表时间：
1977-01-01
期刊：
影响因子：
56.9
通讯作者：
SMITHIES, O
中科院分区：
文献类型：
--
作者：
BLATTNER, FR;WILLIAMS, BG;SMITHIES, O
卡戎开发噬菌体作为克隆载体。它们的构建包含突变，使它们易于使用，并且还大大增加了它们对克隆重组DNA的生物遏制的安全性。当在特殊细菌宿主[大肠杆菌] DP 50 SupF中大量繁殖时，三种Charon载体p53，3A，4A和16 A，被认证可用作EK 2 [生物防护等级2]载体宿主系统。这些系统的安全性进行了评估的一些数据。DNA片段的大小范围为0-2.2 × 10 - 6。在这些EK 2 Charon中可以克隆104个碱基对。
The Charon .lambda. bacteriophages were developed as vectors for cloning. Their construction incorporates mutations that make them simple to use and also greatly increases their safety for the biological containment of cloned recombinant DNA. Three of the Charon vector phages, 3A, 4A and 16A, are certified for use as EK2 [biological containment level 2] vector-host systems, when propagated in bulk in a special bacterial host, [Escherichia coli] DP50SupF. Some data are presented on which the safety of these systems was evaluated. DNA fragments ranging in size from 0-2.2 .times. 104 base pairs can be cloned in these EK2 Charon phages.