AF/R2 adhesin and cytopathic effect as virulence traits of diarrhea-inducing Escherichia coli O103 in European rabbit.
AF/R2 adhesin and cytopathic effect as virulence traits of diarrhea-inducing Escherichia coli O103 in European rabbit.
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AF/R2 粘附素和细胞病变效应作为欧洲兔腹泻诱导大肠杆菌 O103 的毒力特征。
DOI：
10.1007/978-1-4899-1828-4_44
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发表时间：
1997
影响因子：
--
通讯作者：
A. Milon
中科院分区：
文献类型：
--
作者：
C. Chalareng;F. Pillien;M. Boury;C. Tasca;J. De Rycke;A. Milon
属于0103:K-:H2血清型和鼠李糖阴性生物变种的大肠杆菌菌株是西欧国家育种单位断奶兔经常出现危及生命的腹泻的原因（Blanco等人，1994；Camguilhem和A. million, 1989）。根据其致病机制，这些菌株可能被认为是肠致病性大肠杆菌（EPEC）样。它们通过一种叫做AF/R2 （Adhesive Factor/Rabbit 2）的粘附素粘附在肠刷状边缘和HeLa细胞上（Milon et al., 1990）。它们不产生已知毒素（即ST、LT、SLT、CNF、CLDT） （Blanco等人，1994；Mariani-Kurkdjian等人，1993），并具有与EPEC eaeA同源的序列（Leroy等人，1994）。最近，一种新的表型已被确定为基于体外试验，其中包括细菌和HeLa细胞之间的短暂接触，然后在庆大霉素存在下冲洗和孵育细胞。几天后，经0103菌株处理的细胞出现细胞病变效应（CPE）：巨细胞出现具有重应激纤维的细胞质网络。
Escherichia coli strains belonging to 0103:K-:H2 serovar and rhamnose negative biovars are responsible for frequent life-threatening diarrheas in weaned rabbits from national breeding units in western Europe (Blanco et al., 1994; Camguilhem and A. Milon, 1989). According to their mechanisms of pathogenesis, these strains may be considered as enteropathogenic E. coli (EPEC)-like. They adhere to intestinal brush border and to HeLa cells by mean of an adhesin called AF/R2 (Adhesive Factor/Rabbit 2) (Milon et al., 1990). They do not produce known toxins (i.e. ST, LT, SLT, CNF, CLDT) (Blanco et al., 1994; Mariani-Kurkdjian et al., 1993) and bear sequences homologous to EPEC eaeA (Leroy et al., 1994). Recently, a new phenotype has been identified based on an in vitro test that includes a short contact between bacterias and HeLa cells, followed by washes and incubation of cells in presence of gentamicin. After several days, cells treated by 0103 strains develop a cytopathic effect (CPE): apparition of giant cells with a heavy stress fibers cytoplasmic network.