Quantitative trait loci responsible for variation in sexually dimorphic traits in Drosophila melanogaster.
Quantitative trait loci responsible for variation in sexually dimorphic traits in Drosophila melanogaster.
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导致果蝇性二态性状变异的数量性状基因座。
DOI：
10.1093/genetics/163.2.771
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发表时间：
2003
期刊：
影响因子：
3.3
通讯作者：
Nuzhdin,SergeyV
中科院分区：
文献类型：
--
作者：
Kopp,Artyom;Graze,RitaM;Xu,Shizhong;Carroll,SeanB;Nuzhdin,SergeyV
要理解形态进化和物种分化的机制，必须阐明自然群体变异的遗传基础。性二型性状在物种内部和物种之间迅速进化，为解决这些问题提供了有吸引力的模型。在这份报告中，我们地图的数量性状基因座（QTL）的变化，性二型性状（腹部色素沉着和腹侧腹部刚毛和性别梳齿的数量）在一个自然种群ofDrosophila melanogaster.To捕捉的遗传变异模式，目前在野生环境中，一个面板的重组近交系创建从两个杂合苍蝇直接从自然界。细胞学标记的平均密度为每2厘米1个，从而实现了高分辨率绘图。我们使用了一种新的贝叶斯算法，允许QTL定位的基础上，所有的标记同时进行。通过这种方法，我们能够检测到在单标记分析中不明显的小效应QTL。我们的研究结果表明，至少在一些性二型性状，少数QTL占大多数的遗传变异。三个最强的QTL解释了>60%的腹侧刚毛数量的变异。引人注目的是，一个单一的QTL占近60%的女性腹部色素沉着的变化。该QTL定位于Robertsonet al.发现的影响其他种群雌性黑腹果蝇腹部色素沉着的染色体区域。黑腹果蝇使用定量互补试验，我们证明，这个QTL是等位基因的bracgene，其表达以前已被证明与种间差异的色素沉着。赋予不同表型的Multiplebaballeles似乎在自然种群中以可观的频率分离，这表明腹部色素沉着的种内和种间变异可能具有相似的遗传基础。
To understand the mechanisms of morphological evolution and species divergence, it is essential to elucidate the genetic basis of variation in natural populations. Sexually dimorphic characters, which evolve rapidly both within and among species, present attractive models for addressing these questions. In this report, we map quantitative trait loci (QTL) responsible for variation in sexually dimorphic traits (abdominal pigmentation and the number of ventral abdominal bristles and sex comb teeth) in a natural population ofDrosophila melanogaster.To capture the pattern of genetic variation present in the wild, a panel of recombinant inbred lines was created from two heterozygous flies taken directly from nature. High-resolution mapping was made possible by cytological markers at the average density of one per 2 cM. We have used a new Bayesian algorithm that allows QTL mapping based on all markers simultaneously. With this approach, we were able to detect small-effect QTL that were not evident in single-marker analyses. Our results show that at least for some sexually dimorphic traits, a small number of QTL account for the majority of genetic variation. The three strongest QTL account for >60% of variation in the number of ventral abdominal bristles. Strikingly, a single QTL accounts for almost 60% of variation in female abdominal pigmentation. This QTL maps to the chromosomal region that Robertsonet al. have found to affect female abdominal pigmentation in other populations ofD. melanogaster. Using quantitative complementation tests, we demonstrate that this QTL is allelic to thebric a bracgene, whose expression has previously been shown to correlate with interspecific differences in pigmentation. Multiplebaballeles that confer distinct phenotypes appear to segregate in natural populations at appreciable frequencies, suggesting that intraspecific and interspecific variation in abdominal pigmentation may share a similar genetic basis.