RESTRICTED DISTRIBUTION OF INTEGRIN BETA-6 MESSENGER-RNA IN PRIMATE EPITHELIAL TISSUES
RESTRICTED DISTRIBUTION OF INTEGRIN BETA-6 MESSENGER-RNA IN PRIMATE EPITHELIAL TISSUES
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DOI：
10.1177/41.10.8245410
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发表时间：
1993-10-01
影响因子：
3.2
通讯作者：
PYTELA, R
中科院分区：
文献类型：
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作者：
BREUSS, JM;GILLETT, N;PYTELA, R
整合素是介导细胞-细胞外基质和细胞-细胞相互作用的细胞粘附受体。每个整合素由两个糖蛋白亚基（α和β）组成。我们之前已经描述了一种新型整合素β亚基β 6，它在培养的上皮细胞中表达。β 6可以与α v结合形成纤连蛋白结合异源二聚体α v β 6。在这里，我们报告的β 6整合素mRNA的组织分布测定的β 6 cRNA探针与代表性的冷冻组织切片从恒河猴组织库的原位杂交。我们只在上皮细胞中检测到β 6 mRNA。然而，β 6 mRNA的表达在不同的上皮细胞中差异很大。高水平的β 6 mRNA被发现只在两个非常专门的上皮细胞类型：一部分肾小管上皮细胞，称为致密斑，和子宫内膜上皮细胞的分泌期子宫。在子宫内膜中，β 6表达在功能层腺体的分化上皮中最高，表明β 6表达可以以分化依赖性方式进行调节。β 6表达也可能取决于动情周期的阶段，因为我们发现增殖期子宫内膜样本中β 6 mRNA水平低得多。在其他几种组织中的上皮，包括唾液腺导管、胆囊和附睾，含有可检测水平的β 6 mRNA，尽管远低于致密斑和子宫内膜。在其他上皮细胞中，包括皮肤和肺，β 6 mRNA检测不到。综上所述，这些结果表明，在正常成年灵长类动物中，β 6的表达以细胞类型特异性的方式进行调节，仅限于少数上皮组织。
Integrins are cell adhesion receptors that mediate cell-extracellular matrix and cell-cell interactions. Each integrin consists of two glycoprotein subunits (alpha and beta). We have previously described a novel integrin beta-subunit, beta6, which is expressed in cultured epithelial cells. Beta6 can associate with alphav to form the fibronectin-binding heterodimer alphavbeta6. Here we report the tissue distribution of beta6 integrin mRNA determined by in situ hybridization of a beta6 cRNA probe with representative frozen tissue sections from a rhesus monkey tissue bank. We detected beta6 mRNA exclusively in epithelial cells. However, beta6 mRNA expression varied greatly among different epithelia. High levels of beta6 mRNA were found only in two very specialized epithelial cell types: a portion of the kidney tubule epithelium, termed macula densa, and the endometrial epithelium of secretory phase uterus. In the endometrium, beta6 expression was highest in the differentiated epithelium of functional layer glands, suggesting that beta6 expression can be regulated in a differentiation-dependent manner. Beta6 expression may also depend on the stage in the estrous cycle, since we found much lower beta6 mRNA levels in a specimen of proliferative phase endometrium. Epithelium in several other tissues, including salivary gland ducts, gall bladder, and epididymis, contained detectable levels of beta6 mRNA, albeit much lower than in macula densa and endometrium. In other epithelia, including skin and lung, beta6 mRNA was undetectable. Taken together, these results suggest that in normal adult primates beta6 expression is regulated in a cell type-specific manner, restricted to a few epithelial tissues.