Elevated NADPH-oxidase activity in neutrophils from bile-duct-ligated rats: changes in the kinetic parameters and in the oxidase cytosolic factor p47.
Elevated NADPH-oxidase activity in neutrophils from bile-duct-ligated rats: changes in the kinetic parameters and in the oxidase cytosolic factor p47.
复制标题
胆管结扎大鼠中性粒细胞中 NADPH 氧化酶活性升高：动力学参数和氧化酶胞质因子 p47 的变化。
DOI：
10.1016/0167-4889(94)90147-3
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
F. Schlaeffer
中科院分区：
文献类型：
--
作者：
R. Levy;O. Nagauker;E. Sikuler;T. Leto;F. Schlaeffer
Stimulated superoxide generation was 2-fold higher in neutrophils from 20 rats with common bile-duct ligation (CBDL) compared to that of 20 sham-operated control rats. In order to study the mechanism of the higher NADPH oxidase activity in CBDL rats, the kinetic parameters of NADPH oxidase were analyzed. TheVmaxof the NADPH oxidase in CBDL rat neutrophils was significantly higher than that of control rat neutrophils (10.2 and 5.3 nmol/min, respectively). The membrane and cytosol fractions of the oxidase were studied in a cell-free system. Neutrophil cytosol from CBDL rats added to neutrophil membranes from either CBDL or control rats produced 22.4 ± 1.6 and 21.0 ± 1.4nmol/106cells per 10 min, respectively. When neutrophil cytosol from control rats was mixed with neutrophil membranes from control or CBDL rats the generation of superoxide was 10.6 ± 1.4 and 10.0 ± 1.5 nmol/106cells per 10 min, respectively. These results suggest that the cytosol components of the oxidase regulate its activity. By immunoblot analysis it was shown that the amount of the cytosolic factor p47 in neutrophils of CBDL rats is higher than that present in an equal number of neutrophils from control rats.
影响因子：
15.9
作者：
BABIOR, BM
通讯作者：
BABIOR, BM
脂多糖激活小鼠腹腔巨噬细胞会改变产生超氧化物的 NADPH 氧化酶的动力学参数。
DOI：
--
发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Sasada,M;Pabst,MJ;JohnstonJr,RB
通讯作者：
JohnstonJr,RB
影响因子：
4.1
作者：
SEGAL, AW;WEST, I;SCRACE, G
通讯作者：
SCRACE, G