EFFECTS OF 30-PERCENT INTESTINAL RESECTION ON WHOLE POPULATION CELL-KINETICS OF MOUSE INTESTINAL EPITHELIUM
EFFECTS OF 30-PERCENT INTESTINAL RESECTION ON WHOLE POPULATION CELL-KINETICS OF MOUSE INTESTINAL EPITHELIUM
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DOI：
10.1002/ar.1092150106
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发表时间：
1986-05-01
影响因子：
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通讯作者：
BJERKNES, M
中科院分区：
文献类型：
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作者：
CHENG, H;MCCULLOCH, C;BJERKNES, M
切除后残留的肠经历了众所周知的代偿反应。隐窝和绒毛的大小增加，并且隐窝中增殖细胞的数量增加。然而，隐窝标记指数不变，这被认为表明生长分数也保持不变，因此系统扩大，但除此之外，新的稳态与对照相似。也普遍认为，适应性肠中没有新的隐窝或绒毛。在这项研究中，我们应用最近开发的工具来研究整个肠上皮的反应。因此，30%肠切除对整个群体细胞动力学的影响，通过使用流式细胞术，库尔特颗粒计数，和简单的形态测定技术。除了经典的反应，我们发现在隐窝生产的速度增加，这主要是由于一个较短的隐窝复制周期。因此，新的隐窝在切除动物比横切对照组更快的速度产生。这导致切除后上皮中隐窝细胞群的扩增。有一个相应的扩展的循环细胞群，因此在切除的上皮细胞的生长分数增加。我们的结论是，对于隐窝人口，经典的故事是正确的，除了新的隐窝被添加到上皮切除后。然而，对于上皮作为一个整体，经典的故事是误导性的，因为似乎有一个在肠切除后上皮细胞的生长分数增加。
The intestine remaining after resection undergoes a well known compensatory response. Crypts and villi grow in size, and the number of proliferating cells in a crypt increases. The crypt labeling index, however, is unchanged, which is thought to suggest that the growth fraction also remains unchanged and hence that the system is enlarged, but otherwise the new steady-state is similar to that of the controls. It is also generally accepted that no new crypts or villi are added to the adapting bowel. In this study we applied recently developed tools to study the response of the intestinal epithelium as a whole. Thus, the effects of 30% intestinal resection on whole population cell kinetics were determined by using flow cytometry, Coulter particle counting, and simple morphometric techniques. In addition to the classic response, we found an increase in the rate of crypt production, which was due mainly to a shorter crypt replication cycle. Thus, new crypts were produced at a faster rate in the resected animals than in the transected controls. This resulted in an expansion of the crypt cell population in the epithelium following resection. There was a corresponding expansion of the cycling cell population and thus an increase in the growth fraction of the resected epithelium. We conclude that for the crypt population, the classic story is correct with the exception that new crypts are added to the epithelium after resection. However, for the epithelium as a whole, the classic story is misleading as there appears to be an increase in the growth fraction of the epithelium after intestinal resection.