The protective effect of berberine on β-cell lipoapoptosis
The protective effect of berberine on β-cell lipoapoptosis
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DOI：
10.1007/bf03347042
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发表时间：
2011-02-01
影响因子：
5.4
通讯作者：
Li, X-J
中科院分区：
文献类型：
--
作者：
Gao, N.;Zhao, T-Y;Li, X-J
目的：评价黄连素对棕榈酸酯诱导的胰岛β细胞脂凋亡的保护作用，并探讨其可能的作用机制。材料和方法：将HIT-T15胰岛β细胞分为以下处理组：未处理对照组；100 mU M黄连素；0.5 mM棕榈酸酯；0.5 mM棕榈酸酯+0.1 mU M黄连素；0.5 mM棕榈酸酯+1 mU M黄连素；0.5 mM棕榈酸酯+10 mU M黄连素；0.5 mM棕榈酸酯+100 mU M黄连素。48h后用流式细胞仪和Hoechst 33258荧光分光光度法检测细胞凋亡率。用放射免疫法测定基础胰岛素和葡萄糖刺激后的胰岛素水平。采用实时定量聚合酶链式反应和免疫细胞化学法分别检测PPAR-γ的mRNA和蛋白水平。结果：与未治疗的对照组相比，棕榈酸酯治疗组的细胞凋亡显著增加(p
Aims: To evaluate the protective effect of berberine on beta-cell lipoapoptosis induced by palmitate and to explore the possible underlying mechanisms. Materials and methods: HIT-T15 pancreatic beta-cells were divided into the following treatment groups: untreated controls; 100 mu M berberine; 0.5 mM palmitate; 0.5 mM palmitate + 0.1 mu M berberine; 0.5 mM palmitate + 1 mu M berberine; 0.5 mM palmitate + 10 mu M berberine; and 0.5 mM palmitate + 100 mu M berberine. After 48 h, cell apoptosis was assessed by flow cytometry and the Hoechst 33258 fluorescent assay. Basal and glucose-stimulated insulin levels in culture medium were measured by radioimmunoassay. Peroxisome proliferator-activated receptor-gamma(PPAR-gamma) mRNA and protein levels were determined by real-time PCR and immunocytochemistry, respectively. Results: Apoptosis was significantly increased upon treatment with palmitate as compared to the untreated controls (p