ISOLATION AND SEQUENCE-ANALYSIS OF A CDNA CLONE ENCODING A TYPE-1 PROTEIN PHOSPHATASE CATALYTIC SUBUNIT - HOMOLOGY WITH PROTEIN PHOSPHATASE-2A
ISOLATION AND SEQUENCE-ANALYSIS OF A CDNA CLONE ENCODING A TYPE-1 PROTEIN PHOSPHATASE CATALYTIC SUBUNIT - HOMOLOGY WITH PROTEIN PHOSPHATASE-2A
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DOI：
10.1016/0014-5793(87)80316-2
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发表时间：
1987-11-02
期刊：
影响因子：
3.5
通讯作者：
COHEN, PTW
中科院分区：
文献类型：
--
作者：
BERNDT, N;CAMPBELL, DG;COHEN, PTW
从λ gt 10中构建的兔骨骼肌cDNA文库中分离到一个1.5kb的克隆，该克隆含有1型蛋白磷酸酶催化亚基的全长编码序列。从cDNA推导的蛋白质序列包含311个残基，分子量为35.4 kDa。通过北方印迹法观察到1.6 kb的单个mRNA种类。1型蛋白磷酸酶与蛋白磷酸酶2A具有惊人的同源性，在残基11和280之间有49%的氨基酸是相同的。前10个和后31个残基是不同的。1型蛋白磷酸酶的残基1-101也显示与哺乳动物碱性磷酸酶的区域的21%的序列同一性。
A 1.5 kb clone containing the full-length coding sequence of a type-1 protein phosphatase catalytic subunit has been isolated from a rabbit skeletal muscle cDNA library constructed in λgt10. The protein sequence deduced from the cDNA contains 311 residues and has a molecular mass of 35.4 kDa. A single mRNA species at 1.6 kb was visualized by Northern blotting. The type-1 protein phosphatase was strikingly homologous to protein phosphatase 2A, 49% of the amino acids between residues 11 and 280 being identical. The first 10 and last 31 residues were dissimilar. Residues 1–101 of the type-1 protein phosphatase also showed 21% sequence identity with a region of mammalian alkaline phosphatases.