Phosphorus Solubilizing and Releasing Bacteria Screening from the Rhizosphere in a Natural Wetland
Phosphorus Solubilizing and Releasing Bacteria Screening from the Rhizosphere in a Natural Wetland
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DOI：
10.3390/w10020195
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发表时间：
2018-02-01
期刊：
影响因子：
3.4
通讯作者：
Hu, Zhixin
中科院分区：
文献类型：
--
作者：
Cao, Ying;Fu, Dafang;Hu, Zhixin
从天然湿地根际土壤中提取细菌，分别在磷酸三钙或卵磷脂培养基上培养，分离出无机磷(P)溶解菌（IPSB）和有机磷矿化菌（OPMB）。无机磷的增溶是磷有效性的主要贡献，因为分离的细菌从无机磷酸三钙中释放的有效磷比卵磷脂多得多。IPSB 5号在96 h内的P释放率最高，为0.53 mg. l . 1 h(-1)， R10在10 d内的P释放率为0.52 mg. l . 1 h（-1）。鉴定为假单胞菌属和knackmussii假单胞菌。R10的释放量高达125.88毫克。L-1溶出磷酸三钙培养基中的磷，R4溶出有机磷最多，浓度为1.88 mg。10 d内释放率为0.0078 mg.L-1.h（-1）。磷释放量随pH值的降低而增加，只有无机磷释放量随pH值的降低而增加，而有机卵磷脂释放量随pH值的降低而增加。高通量测序结果显示，湿地根际优势菌门为酸杆菌门（Acidobacteria）、变形菌门（Proteobacteria）、拟杆菌门（Bacteroidetes）和绿杆菌门（Chloroflexi），占比分别为34.9%、34.2%、8.8%和4.8%。
Inorganic phosphorus (P)-solubilizing bacteria (IPSB) and organic P-mineralizing bacteria (OPMB) were isolated from bacteria that were first extracted from the rhizosphere soil of a natural wetland and then grown on either tricalcium phosphate or lecithin medium. The solubilizing of inorganic P was the major contribution to P availability, since the isolated bacteria released much more available P from inorganic tricalcium phosphate than lecithin. IPSB No. 5 had the highest P release rate, that is, 0.53 mg.L-1.h(-1) in 96 h, and R10's release rate was 0.52 mg.L-1.h(-1) in 10 days. The bacteria were identified as Pseudomonas sp. and Pseudomonas knackmussii, respectively. R10 released as much as 125.88 mg.L-1 dissolved P from tricalcium phosphate medium, while R4 released the most dissolved P from organic P medium among the isolates, with a concentration of 1.88 mg.L-1 and a releasing rate of 0.0078 mg.L-1.h(-1) in ten days. P releasing increased with a pH decrease only when it was from inorganic P, not organic lecithin, and there was no significant correlation between the culture pH and P solubilizing. High-throughput sequencing analysis revealed that the dominant phylum in the studied wetland rhizosphere consisted of Acidobacteria, Proteobacteria, Bacteroidetes and Chloroflexi, accounting for 34.9%, 34.2%, 8.8% and 4.8%, respectively.