Enhanced translocation and growth of Rhodococcus erythropolis PR4 in the alkane phase of aqueous-alkane two phase cultures were mediated by GroEL2 overexpression.
Enhanced translocation and growth of Rhodococcus erythropolis PR4 in the alkane phase of aqueous-alkane two phase cultures were mediated by GroEL2 overexpression.
复制标题
在水性烷烃两期培养物的烷烃相中，红粒球菌PR4的易位和生长是通过GROEL2过表达介导的。
DOI：
10.1264/jsme2.me13158
复制
发表时间：
2014
影响因子：
2.2
通讯作者：
Sunairi M
中科院分区：
文献类型：
--
作者：
Takihara H;Ogihara J;Yoshida T;Okuda S;Nakajima M;Iwabuchi N;Sunairi M
我们以前报道过R.红串菌PR 4从水相转移到烷烃相，然后在添加了长链烷烃的两相培养物中生长。这被认为有利于生物修复。在本研究中，我们研究了参与R.红串菌PR 4。我们的蛋白基因组学分析的结果表明，GroEL 2的上调更多的细胞内的植烷（C19）相易位比那些位于水-烷烃界面连接到正十二烷（C12）表面。构建PR 4（pK 4-EL 2 -1）和PR 4（pK 4-Δ EL 2 -1）菌株以证实GroEL 2在易位细胞中的上调作用。在含有C12的两相培养中，PR 4（pK 4-EL 2 -1）中GroEL 2的表达是PR 4（pK 4-Δ EL 2 -1）中GroEL 2的表达的15.5倍。通过引入pK 4-EL 2 -1，PR 4的生长和细胞表面亲脂性得到增强。这些结果表明，在PR 4中的groEL 2的质粒过表达（pK 4-EL 2 -1）导致细胞定位的变化、增强的生长和增加的细胞表面亲脂性。因此，我们认为GroEL 2的过表达可能在提高红曲霉对有机溶剂的耐受性中起重要作用。在水-烷烃两相培养物中培养红串菌PR 4。
We previously reported that R. erythropolis PR4 translocated from the aqueous to the alkane phase, and then grew in two phase cultures to which long-chain alkanes had been added. This was considered to be beneficial for bioremediation. In the present study, we investigated the proteins involved in the translocation of R. erythropolis PR4. The results of our proteogenomic analysis suggested that GroEL2 was upregulated more in cells that translocated inside of the pristane (C19) phase than in those located at the aqueous-alkane interface attached to the n-dodecane (C12) surface. PR4 (pK4-EL2-1) and PR4 (pK4-ΔEL2-1) strains were constructed to confirm the effects of the upregulation of GroEL2 in translocated cells. The expression of GroEL2 in PR4 (pK4-EL2-1) was 15.5-fold higher than that in PR4 (pK4-ΔEL2-1) in two phase cultures containing C12. The growth and cell surface lipophilicity of PR4 were enhanced by the introduction of pK4-EL2-1. These results suggested that the plasmid overexpression of groEL2 in PR4 (pK4-EL2-1) led to changes in cell localization, enhanced growth, and increased cell surface lipophilicity. Thus, we concluded that the overexpression of GroEL2 may play an important role in increasing the organic solvent tolerance of R. erythropolis PR4 in aqueous-alkane two phase cultures.