Biological evaluations of zinc hexyl vanillate cement using two in vivo test methods.
Biological evaluations of zinc hexyl vanillate cement using two in vivo test methods.
复制标题
使用两种体内测试方法对己基香草酸锌水泥进行生物学评价。
DOI：
10.1016/s0109-5641(88)80047-2
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Brauer,GM
中科院分区：
文献类型：
--
作者：
Keller,JC;Hammond,BD;Kowalyk,KK;Brauer,GM
通过 2 种方法研究了细胞和组织对 3 种牙科水泥的反应，即 ADA 推荐的结缔组织植入技术 (CTI) 和腹膜腔植入技术 (PCI)，后者已成为定量研究细胞对植入材料的反应的方法。 CTI 研究中检查的所有水泥的大部分时间段都发生由中性粒细胞和巨噬细胞组成的混合炎症细胞反应。使用差异染色技术通过 PCI 研究观察到类似的细胞群，包括淋巴细胞。两种程序的组织病理学观察结果相似。每种水泥都会引起慢性异物反应，其中含有少量慢性细胞和成熟的成纤维细胞活性。根据 CTI 研究中的胶囊厚度和 PCI 研究中的总细胞计数确定，每种水泥观察到的组织反应强度随着时间的增加没有显着差异。对于本工作中使用的两种方法，己基香草酸锌 (ZHV) 水泥的细胞和结缔组织反应与氧化锌-丁子香酚 (ZOE) 和磷酸锌 (ZP) 水泥的反应相似。虽然使用这两种方法在骨水泥植入中获得了类似的组织病理学结果，但 PCI 技术提供了对植入材料的细胞和组织反应的更彻底的研究。除了组织病理学评估之外，PCI 技术还可以对植入物反应的特定细胞进行定量研究，并提供一种使用化学分析技术来量化回收细胞和宿主组织内特定降解产物浓度的机制。最后，这两种方法的结果证明了 ZHV 水泥与临床可接受的 ZP 和 ZOE 水泥配方相比具有可接受的生物学性能。
The cellular and tissue responses to 3 dental cements were studied by 2 methodologies, the connective tissue implantation technique (CTI), recommended by the ADA, and the peritoneal cavity implantation technique (PCI), which has emerged as a method to quantitatively study the cellular response to implanted materials. A mixed inflammatory cell response consisting of neutrophils and macrophages occurred at most of the time periods for all the cements examined in the CTI study. Similar cell populations, including lymphocytes, were observed with the PCI study using differential staining techniques. The histopathological observations from both procedures were similar. Each cement elicited a chronic foreign body reaction containing few chronic cells and mature fibroblastic activity. The intensity of the observed tissue reactions for each cement, as determined by capsule thickness in the CTI study and the overall cell counts in the PCI study, did not differ significantly with increasing time. The cellular and connective tissue reactions to the zinc hexyl vanillate (ZHV) cement approximated those of zinc oxide-eugenol (ZOE) and zinc phosphate (ZP) cements for both methodologies utilized in this work. While similar histopathological results were obtained for the implantation of cements using both methodologies, the PCI technique offers a more thorough investigation of cellular and tissue responses to implanted materials. In addition to histopathological evaluations, the PCI techniques allows quantitative investigation of the specific cells responding to the implants, and provides a mechanism, using chemical analysis techniques, to quantify the concentration of specific degradative products within the retrieved cells and host tissue. Finally, the results from these 2 methodologies demonstrated the acceptable biological performance of ZHV cement compared with the clinically acceptable ZP and ZOE cement formulations.