Transcutaneous oxygen measurement in humans using a paramagnetic skin adhesive film
Transcutaneous oxygen measurement in humans using a paramagnetic skin adhesive film
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DOI：
10.1002/mrm.27445
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发表时间：
2019-02-01
影响因子：
3.3
通讯作者：
Kuppusamy, Periannan
中科院分区：
文献类型：
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作者：
Kmiec, Maciej M.;Hou, Huagang;Kuppusamy, Periannan
目的：经皮氧分压(TcpO(2))提供紧邻皮肤下方组织的血液灌流信息。这些数据在评估伤口愈合问题、诊断外周血管/动脉功能不全以及预测疾病进展或治疗反应方面具有重要价值。目前，TcpO(2)主要是使用电化学皮肤传感器测量的，这种传感器消耗氧气，容易出现校准误差。本研究的目的是建立一种可靠的人体TcpO(2)测量方法。方法：基于电子顺磁共振(EPR)原理，利用氧敏皮肤粘附膜，研制了一种新的TcpO(2)血氧测定方法，称为表面灌注氧分压(SPOT)芯片。SPOT芯片是一个直径3 mm、厚60亩的圆形薄膜，由一个稳定的顺磁氧传感器组成。芯片的一侧覆盖着氧气屏障材料，并通过医用胶带固定在皮肤上，以确保只测量通过皮肤表面扩散的氧气。该方法通过EPR谱线宽对TcpO(2)进行量化。结果：对10名健康受试者的重复测量表明，TcpO(2)测量是稳健的、可靠的和可重复性的。前臂掌侧皮肤TcpO(2)为7.8+/-0.8~22.0+/-1.0 mm Hg(N=29)，足部TcpO(2)为8.1+/-0.3~23.4+/-1.3 mm Hg(N=86)。斑点芯片法可用于临床TcpO(2)的监测。
Purpose: Transcutaneous oxygen tension (TcpO(2)) provides information about blood perfusion in the tissue immediately below the skin. These data are valuable in assessing wound healing problems, diagnosing peripheral vascular/arterial insufficiency, and predicting disease progression or the response to therapy. Currently, TcpO(2) is primarily measured using electrochemical skin sensors, which consume oxygen and are prone to calibration errors. The goal of the present study was to develop a reliable method for TcpO(2) measurement in human subjects.Methods: We have developed a novel TcpO(2) oximetry method based on electron paramagnetic resonance (EPR) principles with an oxygen-sensing skin adhesive film, named the superficial perfusion oxygen tension (SPOT) chip. The SPOT chip is a 3-mm diameter, 60-mu m thick circular film composed of a stable paramagnetic oxygen sensor. The chip is covered with an oxygen-barrier material on one side and secured on the skin by a medical adhesive transfer tape to ensure that only the oxygen that diffuses through the skin surface is measured. The method quantifies TcpO(2) through the linewidth of the EPR spectrum.Results: Repeated measurements using a cohort of 10 healthy human subjects showed that the TcpO(2) measurements were robust, reliable, and reproducible. The TcpO(2) values ranged from 7.8 +/- 0.8 to 22.0 +/- 1.0 mmHg in the volar forearm skin (N = 29) and 8.1 +/- 0.3 to 23.4 +/- 1.3 mmHg in the foot (N = 86).Conclusions: The results demonstrated that the SPOT chip can measure TcpO(2) reliably and repeatedly under ambient conditions. The SPOT chip method could potentially be used to monitor TcpO(2) in the clinic.