Structural flexibility and functional valence of CD4-IgG2 (PRO 542): potential for cross-linking human immunodeficiency virus type 1 envelope spikes.
Structural flexibility and functional valence of CD4-IgG2 (PRO 542): potential for cross-linking human immunodeficiency virus type 1 envelope spikes.
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CD4-IgG2 (PRO 542) 的结构灵活性和功能价：交联人类免疫缺陷病毒 1 型包膜刺突的潜力。
DOI：
10.1128/jvi.75.14.6682-6686.2001
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发表时间：
2001
影响因子：
5.4
通讯作者：
Roux,KH
中科院分区：
文献类型：
--
作者：
Zhu,P;Olson,WC;Roux,KH
CD4-免疫球蛋白G2 （CD4- igg2）将CD4的D1D2结构域的四个拷贝整合到一种抗体样分子中，有效地中和原发性人类免疫缺陷病毒1型。本研究利用电子显微镜研究了CD4-IgG2与gp120配合物的结构和功能价。CD4-γ2是一种二价CD4-免疫球蛋白融合蛋白。CD4-γ - 2 - gp120复合物采用简单的y形结构，而CD4- igg2 - gp120复合物由四个gp120组成，排列在中心CD4- igg2分子周围，这种结构更让人想起补体C1q。分子模型证实了电镜数据，进一步表明CD4- igg2而不是CD4-γ2具有在病毒粒子表面交联gp120-gp41三聚体的显著潜力，提示CD4- igg2抗病毒活性增强的机制。
CD4-immunoglobulin G2 (CD4-IgG2) incorporates four copies of the D1D2 domains of CD4 into an antibody-like molecule that potently neutralizes primary human immunodeficiency virus type 1. Here electron microscopy was used to explore the structure and functional valence of CD4-IgG2 in complex with gp120. CD4-γ2, a divalent CD4-immunoglobulin fusion protein, was evaluated in parallel. Whereas CD4-γ2–gp120 complexes adopted a simple Y-shaped structure, CD4-IgG2–gp120 complexes consisted of four gp120s arrayed about a central CD4-IgG2 molecule, a structure more reminiscent of complement C1q. Molecular modeling corroborated the electron microscopy data and further indicated that CD4-IgG2 but not CD4-γ2 has significant potential to cross-link gp120-gp41 trimers on the virion surface, suggesting a mechanism for the heightened antiviral activity of CD4-IgG2.