Serratia marcescens induces apoptosis in Diaphorina citri gut cells via reactive oxygen species-mediated oxidative stress
Serratia marcescens induces apoptosis in Diaphorina citri gut cells via reactive oxygen species-mediated oxidative stress
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DOI：
10.1002/ps.7787
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发表时间：
2023-10-06
影响因子：
4.1
通讯作者：
Zhang，Ning
中科院分区：
文献类型：
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作者：
Hu，Wei;Zhao，Chongfei;Zhang，Ning
背景亚洲柑橘木虱（Diaphorina citri）是柑橘行业的一种臭名昭著的害虫，因为它传播Candidatus Liberibacterasiaticus，在世界范围内引起一种无法治愈的毁灭性柑橘病害。粘质沙雷氏菌广泛分布在各种环境中，对许多昆虫表现出毒性作用。为了制定提高病原体诱导宿主死亡效率的策略，更好地了解粘质沙雷氏菌柑橘大甲藻的毒性机制至关重要。结果粘质沙雷氏菌KH-001通过喂食人工饲料成功定植柑橘大甲沙雷氏菌，导致细胞核、线粒体、囊泡和微绒毛等细胞损伤。口服粘质沙雷氏菌 KH-001 通过提高 Cyt c、p53 和 caspase-1 的水平并降低细胞凋亡抑制剂 (IAP) 和 Bax 抑制剂-1 (BI-1) 的水平，强烈诱导肠道细胞凋亡。粘质沙雷氏菌 KH-001 上调双氧化酶 (Duox) 和一氧化氮合酶 (Nos) 的表达，从而增加肠道中过氧化氢 (H2O2) 的水平。腹部注射H2O2可加速成虫死亡，并通过激活Cyt c、p53和caspase-1并抑制IAP和BI-1诱导肠道细胞凋亡。用维生素c（Vc）预处理受感染的柑橘大甲虫，可提高成虫存活率并减弱诱导凋亡的作用。结论粘质沙雷氏菌KH-001在柑橘大甲虫肠道内的定植增加了H2O2的积累，导致肠道细胞发生严重变化和凋亡，从而提高了柑橘大甲虫的死亡率。柑橘。本研究确定了粘质沙雷氏菌 KH-001 对柑橘木虱的潜在毒力机制，有助于其在柑橘木虱综合治理中的广泛应用。 © 2023 化学工业协会。
BACKGROUNDAsian citrus psyllid,Diaphorina citri, is a notorious pest in the citrus industry because it transmitsCandidatus Liberibacterasiaticus, which causes an uncurable, devastating disease in citrus worldwide.Serratia marcescensis widely distributed in various environments that exhibits toxic effects to many insects. To develop strategies for enhancing the efficiency of pathogen‐induced host mortality, a better understanding of the toxicity mechanism ofSerratia marcescensonDiaphorina citriis critical.RESULTSSerratia marcescensKH‐001 successfully colonizedDiaphorina citrigut by feeding artificial diets, resulting in the damage of cells including nucleus, mitochondria, vesicles, and microvilli. Oral ingestion ofSerratia marcescensKH‐001 strongly induced apoptosis in gut cells by enhancing levels of Cyt c, p53 and caspase‐1 and decreasing levels of inhibitors of apoptosis (IAP) and Bax inhibitor‐1 (BI‐1). The expression of dual oxidase (Duox) and nitric oxide synthase (Nos) was up‐regulated bySerratia marcescensKH‐001, which increased hydrogen peroxide (H2O2) levels in the gut. Injection of abdomen ofDiaphorina citriwith H2O2accelerated the death of the adults and induced apoptosis in the gut cells by activating Cyt c, p53 and caspase‐1 and suppressing IAP and BI‐1. Pretreatment of infectedDiaphorina citriwith vitamin c (Vc) increased the adult survival and diminished the apoptosis‐inducing effect.CONCLUSIONSThe colonization ofSerratia marcescensKH‐001 in the guts ofDiaphorina citriincreased H2O2accumulation, leading to severe changes and apoptosis in intestinal cells, which enhanced a higher mortality level ofD. citr. This study identifies the underlying virulence mechanism ofSerratia marcescensKH‐001 onDiaphorina citrithat contributes to a widespread application in the integrated management of citrus psyllid. © 2023 Society of Chemical Industry.