Overexpression and RNA interference of Ap-cyclic AMP-response element binding protein-2, a repressor of long-term facilitation, in Aplysia kurodai sensory-to-motor synapses
Overexpression and RNA interference of Ap-cyclic AMP-response element binding protein-2, a repressor of long-term facilitation, in Aplysia kurodai sensory-to-motor synapses
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DOI：
10.1016/s0304-3940(02)01285-5
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发表时间：
2003-01-30
影响因子：
2.5
通讯作者：
Kaang, BK
中科院分区：
文献类型：
--
作者：
Lee, JA;Kim, H;Kaang, BK
环腺苷酸反应元件结合蛋白-2（CREB 2）是CREB/转录因子（CREB/ATF 4）家族的成员。CREB 2是一种转录因子，已知参与失智症长期易化。为了进一步研究ApCREB 2在长时程突触易化中的作用，我们从黑台此外，一个单一的5-HT脉冲，通常只会引起短期的促进，在存在的ApCREB 2抑制RNA干扰，诱导长期的促进在感觉运动突触。这些结果表明，ApCREB 2是一个功能性的阻遏物的长期促进的感觉-运动突触。(C)2002爱思唯尔科学爱尔兰有限公司保留所有权利。
cyclic AMP-response element binding protein-2 (CREB2) is a member of the CREB/transcription factor (CREB/ATF4) family. CREB2 is a transcription factor known to be involved in Aplysia long-term facilitation. To further examine the role of ApCREB2 on long-term synaptic facilitation, we isolated ApCREB2 from Aplysia kurodai in full-length cDNA library, and found that the overexpression of ApCREB2 blocked 5-hydroxytryptamine (5-HT)-induced long-term synaptic facilitation in Aplysia sensory-to-motor synapses. Furthermore, a single pulse of 5-HT, which normally induces only short-term facilitation, in the presence of ApCREB2 inhibition by RNA interference, induced long-term facilitation in Aplysia sensory-to-motor synapses. These results suggest that ApCREB2 is a functional repressor of long-term facilitation in Aplysia sensory-to-motor synapses. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.