Salicylic acid confers resistance to a biotrophic rust pathogen, Puccinia substriata, in pearl millet (Pennisetum glaucum)
Salicylic acid confers resistance to a biotrophic rust pathogen, Puccinia substriata, in pearl millet (Pennisetum glaucum)
复制标题
DOI：
10.1111/j.1364-3703.2008.00532.x
复制
发表时间：
2009-03-01
影响因子：
4.9
通讯作者：
Berger, Dave K.
中科院分区：
文献类型：
--
作者：
Crampton, Bridget G.;Hein, Ingo;Berger, Dave K.
研究了珍珠粟（Pennisetum glaucum）对叶锈菌感染的防御反应途径的诱导。水杨酸（SA）预处理珍珠粟对一种毒力强的锈菌分离株具有抗性，而茉莉酸甲酯（MeJA）没有显著降低侵染水平。这些结果表明，SA防御途径参与了抗锈性。为了鉴定珍珠粟对SA而非MeJA的特异性调控基因，从而在抗性中发挥作用，我们进行了基因表达谱分析。观察到处理之间的基因表达反应存在大量重叠，MeJA和SA处理表现出17%的共调控转录物。然而，34%的转录本在SA处理下出现差异表达，而在MeJA处理下则没有。SA应答转录本代表了参与SA代谢、防御反应、信号转导、氧化应激保护和光合作用的基因。经SA和MeJA处理后，珍珠粟植株的表达谱更为相似，而与草叶假单胞菌侵染时的表达谱更为相似。然而，一些sa反应基因在真菌感染过程中被抑制，这表明可能是病原菌操纵了宿主的反应。
Studies were undertaken to assess the induction of defence response pathways in pearl millet (Pennisetum glaucum) in response to infection with the leaf rust fungus Puccinia substriata. Pretreatment of pearl millet with salicylic acid (SA) conferred resistance to a virulent isolate of the rust fungus, whereas methyl jasmonate (MeJA) did not significantly reduce infection levels. These results suggest that the SA defence pathway is involved in rust resistance. In order to identify pearl millet genes that are specifically regulated in response to SA and not MeJA, and thus could play a role in resistance to P. substriata, gene expression profiling was performed. Substantial overlap in gene expression responses between the treatments was observed, with MeJA and SA treatments exhibiting 17% co-regulated transcripts. However, 34% of transcripts were differentially expressed in response to SA treatment, but not in response to MeJA treatment. SA-responsive transcripts represented genes involved in SA metabolism, defence response, signal transduction, protection from oxidative stress and photosynthesis. The expression profiles of pearl millet plants after treatment with SA or MeJA were more similar to one another than to the response during a compatible infection with P. substriata. However, some SA-responsive genes were repressed during P. substriata infection, indicating possible manipulation of host responses by the pathogen.