Radiosensitivity of different ploidy pollen in poplar and its application

Radiosensitivity of different ploidy pollen in poplar and its application
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发表时间:
2000
期刊:
Acta Genetica Sinica
影响因子:
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通讯作者:
Kang Xy;Zhu Zt;Linford Hb
Kang Xy;Zhu Zt;Linford Hb
中科院分区:
其他
文献类型:
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作者:
Kang Xy;Zhu Zt;Linford Hb

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Through investigation on germinating rate and process of unreduced diploidy pollen and monoploid pollen in Populus tomentosa x P. alba and P. tomentosa, it was proved that there were different radiosensitivities to 60Co gamma-ray among different ploidy pollen, i.e. the radiosensitivity of monoploidy pollen was two times higher than diploidy pollen. To overcome the problems of late germination and poor compatibility of diploidy pollen during fertilization, a certain dose of radiation can be applied to stimulating the diploidy pollen germination, meanwhile to restrain of kill some monoploidy pollen based on their different radiosensibilities. Therefore the rate of germination and faster germinating process of diploidy pollen on stigmas can be increased relatively to certain extent. The efficiency of radiation was proved by breeding practice. In the hybidization of (P. alba x P. glandulosa) x (P. tomentosa x P. bolleana), 3.8% of triploid could be produced by 1,470 rad treatment; while no triploid is examined in the control. 12.9% of triploids were produced by the treatment of 1,680 rad from the above cross.