Acute effects of lactic acid-fermented and enzyme-digested soybean on protein synthesis via mTOR signaling in the skeletal muscle
Acute effects of lactic acid-fermented and enzyme-digested soybean on protein synthesis via mTOR signaling in the skeletal muscle
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乳酸发酵和酶消化的大豆通过骨骼肌中 mTOR 信号对蛋白质合成的急性影响
DOI：
10.1080/09168451.2020.1795810
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Fujino Hidemi
中科院分区：
文献类型：
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作者：
Ono Kohei;Tanaka Minoru;Ikeji Takuya;Nakanishi Ryosuke;Hirabayashi Takumi;Tategaki Airo;Kondo Hiroyo;Ishihara Akihiko;Fujino Hidemi
含有蛋白质的营养素通过增加肌肉蛋白质的合成而导致肌肉的有效肥大。大豆常被运动员或运动人士摄入；然而，它需要很长时间才能被吸收。乳酸发酵酶消化（LFED）大豆比未经处理的大豆吸收更快。本研究旨在测定摄入单丸大豆或由乳酸菌和蛋白酶消化产生的LFED大豆后肌肉蛋白质的合成。8周龄过夜禁食的ICR小鼠分别给予粉状或LFED大豆。小鼠在大豆摄入后7、15、30、60、90和120分钟安乐死。我们已经证明，与在腓肠肌中口服未经处理的大豆相比，LFED大豆通过激活哺乳动物雷帕霉素靶蛋白（mTOR）信号来刺激肌肉蛋白合成的速度更快。这些结果表明，与未经处理的大豆相比，LFED大豆是一种更有效的肌肉肥厚营养来源。
Protein-containing nutrients result in the efficient hypertrophy of muscles by increasing muscle protein synthesis. Soybean is often ingested by athletes or individuals who exercise; however, it takes very long to be absorbed. Lactic acid-fermented and enzyme-digested (LFED) soybean is absorbed faster than untreated soybean. This study aims at determining muscle protein synthesis after ingesting a single bolus of soybean or LFED soybean produced by lactic acid bacteria and protease digestion. Eight-week-old overnight-fasted ICR mice were administered powdered or LFED soybean. Mice were euthanized at 7, 15, 30, 60, 90, and 120 min after soybean intake. We have demonstrated that LFED soybean administration was quicker in stimulating muscle protein synthesis by activating mammalian target of rapamycin (mTOR) signaling than orally ingesting untreated soybean in the gastrocnemius muscle. These results suggested that LFED soybean is a more efficient source of nutrition for muscle hypertrophy than untreated soybean.