Progressive tarsal patterning in the Drosophila by temporally dynamic regulation of transcription factor genes
Progressive tarsal patterning in the Drosophila by temporally dynamic regulation of transcription factor genes
复制标题
通过转录因子基因的时间动态调节果蝇的渐进跗骨模式
DOI：
10.1016/j.ydbio.2011.10.031
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发表时间：
2012
影响因子：
2.7
通讯作者：
Kojima T.
中科院分区：
文献类型：
--
作者：
Natori K.;Tajiri R.;Furukawa S.;Kojima T.
昆虫附肢的形态，如腿部踝节的数量和比例，是非常不同的。在黑腹果蝇中，成年腿有五个颧骨节段。越来越多的证据表明，踝节是在发育过程中通过转录因子基因如Bar基因表达的动态变化而逐渐形成的。在这项研究中，为了进一步研究眼睑进行性模式的基础，我们研究了几个转录因子基因的精确表达模式和功能，以及与Bar表达的时间调控有关的问题。结果表明，Nubbin在早期在较大的区域内表达，但在踝部中部逐渐消失。这导致圆形表达的渐进性扩张，进而逐渐抑制Bar的表达，从而导致眼睑节段3的形成。当启动无翼表达时，形成了对应于睑板片段4的区域，这使得Bar表达难以对圆形表达。此外，颧骨片段2似乎起源于在非常早期阶段表达Bar的区域。在这个区域停止Bar的表达需要无脊椎的功能，这也调节圆形的表达。这些发现表明，这些转录因子基因在时间上的动态调控相互作用是颧骨区域渐进性模式的基本基础。
The morphology of insect appendages, such as the number and proportion of leg tarsal segments, is immensely diverse. In Drosophila melanogaster, adult legs have five tarsal segments. Accumulating evidence indicates that tarsal segments are formed progressively through dynamic changes in the expression of transcription factor genes, such as Bar genes, during development. In this study, to examine further the basis of progressive tarsal patterning, the precise expression pattern and function of several transcription factor genes were investigated in relation to the temporal regulation of Bar expression. The results indicate that nubbin is expressed over a broad region at early stages but gradually disappears from the middle of the tarsal region. This causes the progressive expansion of rotund expression, which in turn progressively represses Bar expression, leading to the formation of the tarsal segment 3. The region corresponding to the tarsal segment 4 is formed when apterous expression is initiated, which renders Bar expression refractory to rotund. In addition, the tarsal segment 2 appears to be derived from the region that expresses Bar at a very early stage. Cessation of Bar expression in this region requires the function of spineless, which also regulates rotund expression. These findings indicate that the temporally dynamic regulatory interaction of these transcription factor genes is the fundamental basis of the progressive patterning of the tarsal region.