Methicillin-resistant Staphylococcus pseudintermedius among dogs admitted to a small animal hospital
Methicillin-resistant Staphylococcus pseudintermedius among dogs admitted to a small animal hospital
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DOI：
10.1016/j.vetmic.2010.12.018
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发表时间：
2011-05-12
影响因子：
3.3
通讯作者：
Nolte, Ingo
中科院分区：
文献类型：
--
作者：
Nienhoff, Ulrike;Kadlec, Kristina;Nolte, Ingo
这项研究的目的是确定17个月期间在一家小型动物医院住院的狗携带耐甲氧西林假中间葡萄球菌(MRSP)的频率，以确定这些分离株的特征，并初步筛选与MRSP携带相关的可能因素。814只狗在进入小型动物医院之前，从鼻/咽和会阴以及伤口和皮肤感染(如果存在)采集拭子。完成了关于背景资料的调查问卷。葡萄球菌的种类和对甲氧西林的耐药性被确认为表型和基因。对鉴定出的MRSP菌株进行SCCmec分型、对25种抗菌药物的药敏试验和SMAL直接脉冲场凝胶电泳。通过单因素列联表和CART分析对携带MRSP的可能危险因素进行了首次筛查，60只狗(7.4%)MRSP阳性。所有MRSP分离株都含有II-III型SCCmec盒，并对抗菌药表现出广泛的耐药性。观察到15种不同的SMAL型。携带MRSP的主要因素是采样前6个月内的住院和抗生素治疗。本研究表明，只有一小部分采样的犬携带多重耐药的MRSP菌株。既往住院和/或抗生素治疗是携带MRSP的潜在相关因素，这一事实突显了在小动物药物中明智使用抗生素的必要性。(C)2010爱思唯尔B.V.保留所有权利。
The aim of this study was to determine the frequency of carriage of methicillin-resistant Staphylococcus pseudintermedius (MRSP) among dogs admitted to a small animal hospital during a 17-month period, to characterize these isolates and to initially screen for possible factors associated with MRSP carriage.Swabs were taken from the nose/pharynx and the perineum as well as from wounds and skin infections (if present) of 814 dogs before entering the small animal hospital. A questionnaire for background information was completed. The staphylococcal species and methicillin resistance were confirmed pheno- and genotypically. The identified MRSP isolates were characterized by SCCmec typing, testing for susceptibility to 25 antimicrobial agents and Smal-directed pulsed-field gel electrophoresis. A first screening for possible risk factors for MRSP carriage was performed by means of unifactorial contingency tables and CART analysis.Sixty (7.4%) dogs were positive for MRSP. All MRSP isolates harboured a type II-III SCCmec cassette and showed extended resistance to antimicrobial agents. Fifteen different Smal patterns were observed. The major factors that clustered with MRSP carriage were former hospitalization and antibiotic treatment within the last six months before sampling.This study showed that only a minor part of the sampled dogs carried multi-resistant MRSP isolates. The facts that prior hospitalization and/or antibiotic therapy are potential associated factors for MRSP carriage underline the necessity of a judicious use of antibiotics in small animal medicine. (C) 2010 Elsevier B.V. All rights reserved.