Staphylococcus aureus bacteriophages mediating the simultaneous lysogenic conversion of beta-lysin, staphylokinase and enterotoxin A: molecular mechanism of triple conversion.
Staphylococcus aureus bacteriophages mediating the simultaneous lysogenic conversion of beta-lysin, staphylokinase and enterotoxin A: molecular mechanism of triple conversion.
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金黄色葡萄球菌噬菌体介导β-赖氨酸、葡萄球菌激酶和肠毒素A同时溶原性转化：三重转化的分子机制。
DOI：
10.1099/00221287-135-6-1679
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
H. Pomeroy
中科院分区：
文献类型：
--
作者：
D. Coleman;D. Sullivan;R. Russell;J. Arbuthnott;B. Carey;H. Pomeroy
发现了一组新的金黄色葡萄球菌血清型F噬菌体，其介导肠毒素A、葡激酶和β-溶素的同时三重溶原性转化。从耐甲氧西林的S. 1971年至1988年间在爱尔兰医院分离的金黄色葡萄球菌和来自菌株PS42-D的金黄色葡萄球菌，该菌株已被用作S.金黄色葡萄球菌分型噬菌体42 D自1965年之前。通过分子克隆、限制性内切酶位点图谱和杂交分析，确定了其中三种噬菌体介导的三重转化的分子机制，并与血清型F介导的β-溶素和葡萄球菌激酶转化机制进行了比较，双转化相ph 13。从三重转化噬菌体DNA中克隆了介导肠毒素A（entA）和葡激酶（sak）表达的遗传决定簇，并在大肠杆菌和S.金黄色。在每种情况下，entA和sak决定簇在邻近噬菌体附着位点（attP）的噬菌体DNA中紧密连接，此外，噬菌体phi 13的sak决定簇也位于其attP附近。三种三重转化酶的含entA、sak和attP的DNA区域的限制性图谱彼此非常相似，并且与噬菌体phi 13的相应的含sak和attP的DNA区域非常相似。使用克隆的β-溶素决定簇（HLB）和克隆的含attP的DNA片段作为探针的杂交分析表明，由三重转化的噬菌体和噬菌体phi 13介导的β-溶素转化是由染色体编码的HLB决定簇的插入失活引起的，所述HLB决定簇通过在溶原化后噬菌体DNA的定向特异性整合而被插入失活。
A new group of serotype F bacteriophages of Staphylococcus aureus has been found which mediates the simultaneous triple-lysogenic conversion of enterotoxin A, staphylokinase and beta-lysin. The phages were recovered fro methicillin-resistant strains of S. aureus isolated in Irish hospitals between 1971 and 1988 and from strain PS42-D, which has been used as the propagating strain for the S. aureus typing phage 42D since before 1965. The molecular mechanism of triple conversion mediated by three of these phages was determined by molecular cloning, restriction endonuclease site mapping and hybridization analysis, and compared with the mechanism of beta-lysin and staphylokinase conversion mediated by the serotype F, double-converting phase phi 13. THe genetic determinants mediating expression of enterotoxin A (entA) and staphylokinase (sak) were cloned from the DNA of the triple-converting phage and expression of the cloned determinants detected in Escherichia coli and S. aureus. The entA and sak determinants were closely linked in the phage DNA adjacent to the phage attachment site (attP) in each case and furthermore, the sak determinant of phage phi 13 was also located near its attP. The restriction maps of the entA-, sak- and attP-containing DNA regions of the three triple-converting phages were very similar to each other and to the corresponding sak- and attP- containing DNA region of phage phi 13. Hybridization analysis using a cloned beta-lysin determinant (hlb) and cloned attP-containing DNA fragments as probes demonstrated that beta-lysin conversion mediated by the triple-converting phages and phage phi 13 was caused by insertional inactivation of the chromosomally encoded hlb determinant by orientation-specific integration of phage DNA following lysogenization.