The sorting sequence of the peroxisomal integral membrane protein PMP47 is contained within a short hydrophilic loop.
The sorting sequence of the peroxisomal integral membrane protein PMP47 is contained within a short hydrophilic loop.
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DOI：
10.1083/jcb.133.2.269
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发表时间：
1996-04
期刊：
影响因子：
--
通讯作者：
Goodman JM
中科院分区：
文献类型：
--
作者：
Dyer JM;McNew JA;Goodman JM
尚未鉴定过氧化物酶体整合膜蛋白的靶向序列。我们以前已经表明，一个67个氨基酸的区域是必需的目标Pmp47，一种跨越膜六次的蛋白质，过氧化物酶体。该区域包括两个膜跨度和中间环。我们现在证明，20个氨基酸的环，这是预测面对的矩阵，是必要的和足够的过氧化物酶体靶向。以氯霉素乙酰转移酶和绿色荧光蛋白为载体，证明了其有效性。在环的中间有一簇碱性氨基酸，我们预测它们通过侧翼茎结构从膜表面突出到基质中。我们表明，靶向信号是由这个基本的集群和一个块的氨基酸直接从它的下游。
No targeting sequence for peroxisomal integral membrane proteins has yet been identified. We have previously shown that a region of 67 amino acids is necessary to target Pmp47, a protein that spans the membrane six times, to peroxisomes. This region comprises two membrane spans and the intervening loop. We now demonstrate that the 20 amino acid loop, which is predicted to face the matrix, is both necessary and sufficient for peroxisomal targeting. Sufficiency was demonstrated with both chloramphenicol acetyltransferase and green fluorescent protein as carriers. There is a cluster of basic amino acids in the middle of the loop that we predict protrudes from the membrane surface into the matrix by a flanking stem structure. We show that the targeting signal is composed of this basic cluster and a block of amino acids immediately down-stream from it.