Apolipoprotein C1 (APOC1): A Novel Diagnostic and Prognostic Biomarker for Cervical Cancer.
Apolipoprotein C1 (APOC1): A Novel Diagnostic and Prognostic Biomarker for Cervical Cancer.
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载脂蛋白 C1 (APOC1)：一种新型宫颈癌诊断和预后生物标志物
DOI：
10.2147/ott.s280690
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发表时间：
2020
影响因子：
4
通讯作者：
Chen Y
中科院分区：
文献类型：
--
作者：
Shi X;Wang J;Dai S;Qin L;Zhou J;Chen Y
研究背景APOC 1与多种肿瘤相关，但其在宫颈癌中的作用尚不清楚。本研究旨在探讨载脂蛋白C 1（APOC 1）在宫颈癌中的临床疗效及功能。材料与方法采用RT-qPCR方法检测APOC 1在宫颈癌组织中的相对表达。为了更准确地检测细胞的增殖、迁移、侵袭能力和凋亡，我们分别采用CCK 8法、Edu法、伤口愈合法、迁移侵袭法、流式细胞仪法、免疫共沉淀法、蛋白质组学和Western blot法沉默和过表达APOC 1。在体外和体内探索了APOC 1对肿瘤进展的作用。结果APOC 1在宫颈癌组织中的相对表达明显上调（P<0.05）。在宫颈癌细胞系中，APOC 1的沉默抑制了细胞的增殖和EMT，而APOC 1的过表达促进了细胞的增殖和EMT（P<0.05）。结论APOC 1在宫颈癌中起癌基因的作用，敲低APOC 1可抑制宫颈癌细胞的生长。APOC 1的相对表达与宫颈癌患者的临床预后密切相关。
Background Previous reports showed that APOC1 was associated with several cancers but the function of APOC1 in cervical cancer was unknown. This study aimed to investigate the clinical effect and function of APOC1 in cervical cancer. Materials and Methods In this study, the relative expression of APOC1 in cervical cancer was detected by RT-qPCR. In order to determine the cell proliferation and migration and invading ability and apoptosis more accurately, we used CCK8 assay, Edu assay, wound healing assay, migration and invasion assay, flow cytometry assay, co-immunoprecipitation, proteomics and Western blot by silencing and overexpressing APOC1, respectively. The role of APOC1 on tumor progression was explored in vitro and vivo. Results The relative expression of APOC1 in cervical cancer tissues was up-regulated (P<0.05). In cervical cancer cell lines, silencing of APOC1 restrained cell progression and EMT, while over-expression of APOC1 accelerated cell progression and EMT in vivo and vitro (P<0.05). Conclusion APOC1 acts as an oncogene in cervical cancers and knockdown of APOC1 inhibited cervical cancer cells growth in vitro and in vivo. There is a close relationship between the relative expression of APOC1 and clinical outcome in cervical cancer patients.