Exosomes from 3D culture of marrow stem cells enhances endothelial cell proliferation, migration, and angiogenesis via activation of the HMGB1/AKT pathway
Exosomes from 3D culture of marrow stem cells enhances endothelial cell proliferation, migration, and angiogenesis via activation of the HMGB1/AKT pathway
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来自骨髓干细胞 3D 培养物的外泌体通过激活 HMGB1/AKT 通路增强内皮细胞增殖、迁移和血管生成
DOI：
10.1016/j.scr.2020.102122
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发表时间：
2021-01-01
影响因子：
1.2
通讯作者：
Xu, Yue
中科院分区：
文献类型：
--
作者：
Gao, Wenling;Liang, Tangzhao;Xu, Yue
背景：血管生成是组织工程的重要步骤。MSC外泌体在血管生成中起重要作用。外泌体中由培养微环境赋予的功能性生物分子可以转移到受体细胞并影响其效果。三维培养可促进MSCs的增殖和活性。然而，来源于骨髓间充质干细胞三维培养的外泌体是否具有增强血管生成的作用尚不清楚。方法：在此，我们比较了骨髓间充质干细胞（BMSCs）常规二维培养（2D-exos）和三维培养（3D-exos）产生的外泌体在血管生成中的生物活性。一系列体外和体内实验表明，3D-exos对HUVEC细胞增殖、迁移、管形成和体内血管生成的影响比2D-exos更强。3D-exos的优势可能是由于HMGB 1/AKT信号的激活。结论：这些结果表明，来自3D培养的MSCs的exosomes可能作为一种潜在的促血管生成的治疗方法。
Background: Angiogenesis is an essential step in tissue engineering. MSC exosomes play an important role in angiogenesis. Functional biomolecules in exosomes vested by the culture microenvironment can be transferred to recipient cells and affects their effect. 3D culture can improve the proliferation and activity of MSCs. However, whether exosomes derived from 3D culture of MSCs have an enhanced effect on angiogenesis is unclear.Methods: Herein, we compared the bioactivity of exosomes produced by conventional 2D culture (2D-exos) and 3D culture (3D-exos) of bone marrow stem cells (BMSCs) in angiogenesis.Results: A series of in vitro and in vivo experiments indicated that 3D-exos exhibited stronger effects on HUVEC cell proliferation, migration, tube formation, and in vivo angiogenesis compared with 2D-exos. Moreover, the superiority of 3D-exos might be attributed to the activation of HMGB1/AKT signaling.Conclusions: These results indicate that exosomes from 3D culture of MSCs may serve as a potential therapeutic approach for pro-angiogenesis.