Magnetometry of ingested particles in pulmonary macrophages.
Magnetometry of ingested particles in pulmonary macrophages.
复制标题
肺巨噬细胞中摄入颗粒的磁力测定。
作者：
P. Valberg
灵敏的磁学测量表明，人类或动物吸入空气中的磁性粉尘后，滞留在肺部的颗粒会旋转。已经提出了许多这种旋转的机制，包括呼吸运动、粒子热能、心脏跳动、体表液体流动和巨噬细胞胞浆运动。在本研究中，通过磁测量和视频显微镜观察吸入磁铁矿(γ-Fe_2O_3)气雾剂1天后从金黄地鼠肺中取出的肺巨噬细胞的细胞机制。在贴壁细胞中测量了磁化后剩余的磁场，发现在12分钟内迅速衰减到其初始幅度的30%。细胞质运动的抑制剂减缓了剩余场的衰变速度。吞噬了伽马-Fe2O的肺巨噬细胞的视频显微镜显示，变形虫运动使颗粒偏离其原始磁化方向。结果证实，巨噬细胞胞浆运动是肺剩余磁场衰减的主要原因，磁测量可以用来量化细胞内的收缩活动。
Sensitive magnetometry has shown that, after inhalation of airborne magnetic dust by humans or animals, particles retained within the lungs rotate. A number of mechanisms for this rotation have been proposed, including motions of breathing, particle thermal energy, cardiac pulsations, surface fluid flows, and macrophage cytoplasmic movements. In this study the cellular mechanism was examined by magnetometry and videomicroscopy of pulmonary macrophages removed from hamster lungs 1 day after inhalation of a maghemite (gamma-Fe2O3) aerosol. The field remaining after magnetization was measured in adherent cells and was found to decay rapidly to 30 percent of its initial magnitude within 12 minutes. The remanent-field decay rate was slowed by inhibitors of cytoplasmic motion. Videomicroscopy of pulmonary macrophages with phagocytized gamma-Fe2O3 showed amoeboid motions that rotated the particles away from their original direction of magnetization. The results confirm that macrophage cytoplasmic movement is a primary cause of remanent-field decay in lungs and that magnetometry can be used to quantify intracellular contractile activity.