Biomolecular motor-driven microtubule translocation in the presence of shear flow: analysis of redirection behaviours
Biomolecular motor-driven microtubule translocation in the presence of shear flow: analysis of redirection behaviours
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DOI：
10.1088/0957-4484/18/2/025101
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发表时间：
2007-01
期刊：
影响因子：
3.5
通讯作者：
Taesung Kim;Ming-Tse Kao;E. Meyhöfer;E. Hasselbrink
中科院分区：
文献类型：
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作者：
Taesung Kim;Ming-Tse Kao;E. Meyhöfer;E. Hasselbrink
我们提出了一个概念，为动力的微流体装置与生物分子马达和微管，以满足高效率的微流体装置的需求。然而，要成功地实现这样的设备，我们需要的方法，主动控制微管易位的方向。虽然大多数以前的工作已经采用了大量的微制造的被动机械图案设计，以指导微管的方向，在本文中，我们证明了流体动力剪切流可以用来对齐微管易位在驱动蛋白涂层的表面上的方向平行于流体流动。我们的证据支持这一假设，微管重定向的机制是简单的，由粘性剪切引起的阻力弯曲的微管，这可能是悬臂超出其驱动蛋白支持的前端。该悬臂端朝向流动方向偏转，直到其随后结合到另外的驱动蛋白;随着易位继续，该过程重复，直到微管在很大程度上与流动对齐，达到由热能产生的随机波动确定的极限。我们目前的统计微管对齐率与各种强度的剪切流以及驱动蛋白的浓度，并探讨剪切流的运动性的影响。
We suggest a concept for powering microfluidic devices with biomolecular motors and microtubules to meet the demands for highly efficient microfluidic devices. However, to successfully implement such devices, we require methods for active control over the direction of microtubule translocation. While most previous work has employed largely microfabricated passive mechanical patterns designed to guide the direction of microtubules, in this paper we demonstrate that hydrodynamic shear flow can be used to align microtubules translocating on a kinesin-coated surface in a direction parallel to the fluid flow. Our evidence supports the hypothesis that the mechanism of microtubule redirection is simply that drag force induced by viscous shear bends the leading end of a microtubule, which may be cantilevered beyond its kinesin supports. This cantilevered end deflects towards the flow direction, until it is subsequently bound to additional kinesins; as translocation continues, the process repeats until the microtubule is largely aligned with the flow, to a limit determined by random fluctuations created by thermal energy. We present statistics on the rate of microtubule alignment versus various strengths of shear flow as well as concentrations of kinesin, and also investigate the effects of shear flow on the motility.