Phylogeny of Streptomyces species and evidence for horizontal transfer of entire and partial antibiotic gene clusters
Phylogeny of Streptomyces species and evidence for horizontal transfer of entire and partial antibiotic gene clusters
复制标题
DOI：
10.1023/a:1010296220929
复制
发表时间：
2001-06-01
影响因子：
2.6
通讯作者：
Wellington, EMH
中科院分区：
文献类型：
--
作者：
Egan, S;Wiener, P;Wellington, EMH
链霉菌土壤分离物和模式菌株的集合的系统发育关系，解决了通过序列分析的trpB，参与色氨酸生物合成的管家基因。分析证实，两个分离株的受体在基因转移事件，证明了trpB和strB1树之间的系统发育不一致。一个菌株获得了整个链霉素生物合成簇，而另一个菌株仅包含strRAB 1、抗性基因和来自该簇的两个侧翼基因。trpB的序列分析，作为多相方法的一部分，是一个有用的工具，在确定属内链霉菌属内的关系。
The phylogenetic relationships of a collection of streptomycete soil isolates and type strains were resolved by sequence analysis of trpB, a housekeeping gene involved in tryptophan biosynthesis. The analysis confirmed that two isolates were recipients in a gene transfer event, demonstrated by phylogenetic incongruency between trpB and strB1 trees. One strain had acquired the entire streptomycin biosynthetic cluster, whilst the other contained only strRAB1, the resistance gene and two flanking genes from the cluster. Sequence analysis of trpB, as part of a polyphasic approach, was a useful tool in determining intra-generic relationships within the genus Streptomyces.