CULTURE OF ARTERIAL CHEMORECEPTOR CELLS FROM ADULT CATS IN DEFINED MEDIUM
CULTURE OF ARTERIAL CHEMORECEPTOR CELLS FROM ADULT CATS IN DEFINED MEDIUM
复制标题
DOI：
10.1016/s0006-8993(09)90011-7
复制
发表时间：
1994-09-26
期刊：
影响因子：
2.9
通讯作者：
GUILARTE, TR
中科院分区：
文献类型：
--
作者：
SHIRAHATA, M;SCHOFIELD, B;GUILARTE, TR
最近，膜片钳技术和光学荧光技术被应用于新鲜分离或培养的颈动脉体球细胞，以研究颈动脉的化学传感机制。尸体。然而，很少有研究表明解离和/或培养条件对细胞的健康和功能的影响。本研究的目的是建立一种支持健康和功能正常的成年猫颈动脉体细胞的培养方法。用0.1~0.2%的胶原酶分离颈动脉小体，轻微摩擦。这些细胞被放置在涂有聚D-赖氨酸和Matrigel的玻璃孔上，并在化学定义的培养液中培养。培养长达37天，未见成纤维细胞过度生长。球体细胞在簇内和簇外延伸它们的突起。单个球体细胞形成神经元的形状。球体细胞合成多巴胺，其分泌在细胞暴露于低氧期间增加。酪氨酸羟化酶在整个培养过程中均有表达。这些结果表明，在本文所述条件下培养的血管球瘤细胞是健康的，其功能与体内相似。
Recently patch clamp techniques and optical fluorometric techniques have been applied to freshly dissociated or cultured carotid body glomus cells to investigate chemosensory mechanisms of the carotid. body. However, very few studies have shown the effects of the dissociation and/or culture conditions on the health and function of the cells. The purpose of this study was to develop a culture method which support healthy and functioning carotid body cells from adult cats. Carotid bodies were dissociated with 0.1-0.2% collagenase and gentle trituration. The cells were plated on glass wells coated with poly-D-lysin and Matrigel, and cultured in chemically defined medium. Culture was maintained for up to 37 days without overgrowth of fibroblasts. Glomus cells extended their processes within and from clusters. Single glomus cells acquired the shape of neurons. Glomus cells synthesized dopamine and its secretion increased during exposure of the cells to hypoxia. Tyrosine hydroxylase was expressed throughout the culture period. These results indicate that glomus cells cultured under conditions described here are healthy and function in a manner similar to that in vivo.