Delineating metabolomic changes in native isolate Aurantiochytrium for production of docosahexaenoic acid in presence of varying carbon substrates
Delineating metabolomic changes in native isolate Aurantiochytrium for production of docosahexaenoic acid in presence of varying carbon substrates
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DOI：
10.1016/j.algal.2021.102285
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发表时间：
2021-03-31
影响因子：
5.1
通讯作者：
Jutur, Pannaga P.
中科院分区：
文献类型：
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作者：
Mariam, Iqra;Kareya, Mukul S.;Jutur, Pannaga P.
富含多不饱和脂肪酸（PUFAs）如二十二碳六烯酸（DHA）的油因其对人体营养和健康的益处而具有工业意义。在此背景下，我们用葡萄糖（即2%，3%,4%,6%，8%和10% w/v）对海洋原生thraustochytrid Aurantiochytrium sp.)进行初步筛选，发现3% （w/v）的浓度是进一步研究的最佳浓度。因此，与其他替代碳底物（如半乳糖、果糖、木糖、蔗糖和甘油）互补对提高生物量和DHA产量的影响进行了评估。结果表明，总生物量和DHA产量在3% （w/v）甘油条件下最大，分别为1.91 g L-1 D-1和134.76 mg L-1 D-1。此外，不同碳底物的利用曲线表明，尽管甘油（0.174 g h-1）的消耗率低于葡萄糖（0.209 g h-1）、半乳糖和果糖（0.208 g h-1），但它的生物量（Yx/s）产量更高，为0.5 g g- 1。进一步通过气相色谱-质谱（GC-MS）进行定性代谢组学研究，鉴定出34种代谢产物，包括脂肪酸、糖和有机酸，这些代谢产物被发现受到显著调节。这导致了我们的假设，甘油可以诱导细胞生长，提高脂肪酸产量通过。，戊糖磷酸途径上调。总之，本研究证明了生物精炼副产物（如粗甘油）的有效增值，作为一种具有成本效益的替代方法，可以从食品、营养保健和制药工业的必需成分异养水牛油中生产高附加值的pufa。
Oils rich in polyunsaturated fatty acids (PUFAs) such as docosahexaenoic acid (DHA) have industrial significance due to their benefits in human nutrition and health. In this context, our preliminary screening with glucose (i.e., 2%, 3%, 4%, 6%, 8% and 10% w/v), in a marine indigenous thraustochytrid Aurantiochytrium sp., revealed that a concentration of 3% (w/v) was the optimal for further study. Henceforth, the impact of complementation to improve the biomass and DHA production were assessed with other alternative carbon substrates like galactose, fructose, xylose, sucrose, and glycerol. Our results demonstrate that the overall biomass and DHA productivities were maximal in 3% (w/v) glycerol, i.e. 1.91 g L-1 D-1, and 134.76 mg L-1 D-1, respectively. Also, utilization profiles of different carbon substrates demonstrate that although glycerol (0.174 g h-1) has a lower consumption rate than glucose (0.209 g h-1), galactose and fructose (0.208 g h-1), it results in higher biomass (Yx/s) yield of 0.5 g g- 1 of the substrate. Further the investigation was done by qualitative metabolomics through gas chromatography-mass spectrometry (GC-MS) identified 34 metabolites comprised of fatty acids, sugars, and organic acids which were found to be significantly regulated. This led to our hypothesis that glycerol can induce cell growth with improved fatty acid yields via., upregulation of pentose phosphate pathway. In conclusion, this study demonstrates efficient valorization of biorefinery by-products (such as crude glycerol) as a cost-effective alternative for the production of high-value added PUFAs from heterotrophic thraustochytrids, the essential ingredients for food, nutraceutical and pharmaceutical industries.