GENETIC-ANALYSIS OF IMMORTALIZING FUNCTIONS OF EPSTEIN-BARR VIRUS IN HUMAN LYMPHOCYTES-B
GENETIC-ANALYSIS OF IMMORTALIZING FUNCTIONS OF EPSTEIN-BARR VIRUS IN HUMAN LYMPHOCYTES-B
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DOI：
10.1038/340393a0
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发表时间：
1989-08-03
期刊：
影响因子：
64.8
通讯作者：
SUGDEN, B
中科院分区：
文献类型：
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作者：
HAMMERSCHMIDT, W;SUGDEN, B
爱泼斯坦-巴尔病毒(EBV)是一种疱疹病毒，在体外感染人类B淋巴细胞并有效地使其永生。EBV的大约100个基因中约有10个在最近永生化的B细胞中表达，尽管有间接证据表明其中至少有3个可能与永生化过程有关，但没有直接证据表明需要任何特定的基因2。我们已经开发出一种EBV的遗传分析方法，它使用转化缺陷的病毒株作为辅助病毒，并结合包含病毒生命周期裂解阶段复制、切割和包装所需的所有顺式作用元件的DNA。这种DNA可以包括永生所需的病毒基因，以补充转化缺陷病毒株。DNA可以被辅助病毒的产物扩增和包装，包装后的DNA具有感染性。我们分析了在永生化细胞中表达的两个病毒基因，发现编码EBV核抗原-2的基因是永生化所必需的，而EBV核抗原前导蛋白基因则不是。
EPSTEIN–BARR virus (EBV), a herpes virus, infects human B lymphocytesin vitroand efficiently immortalizes them1. About 10 of the approximately 100 genes of EBV are expressed in recently immortalized B cells and although there is circumstantial evidence that at least three of these may contribute to the process of immortalization, there is no direct evidence that any particular gene is required2. We have developed a genetic analysis of EBV that uses a transformation-defective strain of the virus as a helper virus in conjunction with DNA that contains all of the viralcis-acting elements required for replication, cleavage and packaging during the lytic phase of the viral life cycle. This DNA can include viral genes required for immortalization that complement the transformation-defective virus strain. The DNA can be amplified and packaged by the products of the helper virus and the packaged DNA is infectious. We have analysed two viral genes expressed in immortalized cells and find that the gene encoding EBV nuclear antigen-2 is required for immortalization, whereas the gene for the EBV nuclear antigen leader protein is not.