Noninvasive in situ evaluation of osteogenic differentiation by time-resolved laser-induced fluorescence spectroscopy
Noninvasive in situ evaluation of osteogenic differentiation by time-resolved laser-induced fluorescence spectroscopy
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DOI：
10.1089/107632704323061771
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发表时间：
2004-03-01
影响因子：
--
通讯作者：
Hedrick, MH
中科院分区：
文献类型：
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作者：
Ashjian, P;Elbarbary, A;Hedrick, MH
生物工程组织的临床植入需要在移植前对体外开发的组织构建体的质量进行原位无损评估。时间分辨激光诱导荧光光谱 (TR-LIFS) 在此被证明可以无创地监测由源自人类脂肪组织的假定干细胞 (PLA 细胞) 产生的成骨细胞外基质 (ECM) 的形成。我们表明，这种光谱技术可以评估新形成的成骨 ECM 中胶原蛋白（I、III、IV 和 V 型）的相对表达。结果与传统组织化学技术（免疫荧光和蛋白质印迹）获得的结果一致，并证明 TR-LIFS 是监测完整组织结构中不同胶原类型表达和胶原交联形成的潜在工具。
The clinical implantation of bioengineered tissues requires an in situ nondestructive evaluation of the quality of tissue constructs developed in vitro before transplantation. Time-resolved laser-induced fluorescence spectroscopy (TR-LIFS) is demonstrated here to noninvasively monitor the formation of osteogenic extracellular matrix (ECM) produced by putative stem cells (PLA cells) derived from human adipose tissue. We show that this optical spectroscopy technique can assess the relative expression of collagens (types I, III, IV, and V) within newly forming osteogenic ECM. The results are consistent with those obtained by conventional histochemical techniques (immunofluorescence and Western blot) and demonstrate that TR-LIFS is a potential tool for monitoring the expression of distinct collagen types and the formation of collagen cross-links in intact tissue constructs.