Assignment and NOE analysis of 2′-hydroxyl protons in RNA:: Implications for stabilization of RNA A-form duplexes
Assignment and NOE analysis of 2′-hydroxyl protons in RNA:: Implications for stabilization of RNA A-form duplexes
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DOI：
10.1021/ja043390o
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发表时间：
2005-02-23
影响因子：
15
通讯作者：
Carlomagno, T
中科院分区：
文献类型：
--
作者：
Hennig, M;Fohrer, J;Carlomagno, T
核糖2 '-OH羟基将RNA与DNA区分开来。2 '-OH羟基质子负责RNA和DNA寡核苷酸的构象、水合和热力学稳定性的差异。此外，2 '-OH基团在RNA催化中起着核心作用。这个重要的基团位于RNA的浅沟中，在那里它与水分子形成氢键网络，稳定RNA A型双链体。关于2 '-OH羟基质子的结构和动力学信息对于理解它们各自的作用是必不可少的。在这里，我们报告的2 '-OH羟基质子分配的30聚体RNA，HIV-2的反式激活区域，在水中使用溶液NMR技术。我们提供了2 '-OH羟基的结构信息，在取向偏好的形式相矛盾的范式，2'-OH羟基通常指向远离核糖H1'质子。
The ribose 2‘-OH hydroxyl group distinguishes RNA from DNA. The 2‘-OH hydroxyl protons are responsible for differences in conformation, hydration, and thermodynamic stability of RNA and DNA oligonucleotides. Additionally, the 2‘-OH group plays a central role in RNA catalysis. This important group lies in the shallow groove of RNA, where it is involved in a network of hydrogen bonds with water molecules stabilizing RNA A-form duplexes. Structural and dynamical information on 2‘-OH hydroxyl protons is essential to understand their respective roles. Here we report the 2‘-OH hydroxyl proton assignments for a 30mer RNA, the HIV-2 transactivation region, in water using solution NMR techniques. We provide structural information on 2‘-OH hydroxyl groups in the form of orientational preferences contradicting the paradigm that the 2‘-OH hydroxyl typically points away from the ribose H1‘ proton.