The mammalian circadian pacemaker regulates wakefulness via CRF neurons in the paraventricular nucleus of the hypothalamus.
The mammalian circadian pacemaker regulates wakefulness via CRF neurons in the paraventricular nucleus of the hypothalamus.
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DOI：
10.1126/sciadv.abd0384
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发表时间：
2020-11
期刊：
影响因子：
13.6
通讯作者：
Yamanaka A
中科院分区：
文献类型：
--
作者：
Ono D;Mukai Y;Hung CJ;Chowdhury S;Sugiyama T;Yamanaka A
下丘脑神经元回路介导小鼠昼夜节律调节的觉醒。在哺乳动物中，生理功能的日常节律由位于下丘脑视交叉上核（SCN）的中央生物钟计时。尽管 SCN 对于调节睡眠/觉醒的重要性已经被提出，但人们对 SCN 调节睡眠/觉醒的神经元投射知之甚少。在这里，我们发现下丘脑室旁核中的促肾上腺皮质激素释放因子（CRF）神经元介导 SCN 的昼夜节律并调节觉醒。 CRF 神经元的光遗传学激活通过下丘脑外侧的食欲素/下丘脑分泌素神经元促进觉醒。体内 Ca2+ 记录显示 CRF 神经元在觉醒开始时活跃。此外，CRF 神经元的化学遗传学抑制和消融减少了运动活动和清醒时间。最后，光学操纵和 Ca2+ 成像相结合揭示了 CRF 神经元的神经元活动受到 SCN 中 GABA 能神经元的负调节。我们的研究结果为哺乳动物睡眠/觉醒的昼夜节律调节提供了重要的见解。
A hypothalamic neuronal circuit mediates circadian-regulated wakefulness in mice. In mammals, the daily rhythms of physiological functions are timed by the central circadian clock located in the suprachiasmatic nucleus (SCN) of the hypothalamus. Although the importance of the SCN for the regulation of sleep/wakefulness has been suggested, little is known about the neuronal projections from the SCN, which regulate sleep/wakefulness. Here, we show that corticotropin-releasing factor (CRF) neurons in the hypothalamic paraventricular nucleus mediate circadian rhythms in the SCN and regulate wakefulness. Optogenetic activation of CRF neurons promoted wakefulness through orexin/hypocretin neurons in the lateral hypothalamus. In vivo Ca2+ recording showed that CRF neurons were active at the initiation of wakefulness. Furthermore, chemogenetic suppression and ablation of CRF neurons decreased locomotor activity and time in wakefulness. Last, a combination of optical manipulation and Ca2+ imaging revealed that neuronal activity of CRF neurons was negatively regulated by GABAergic neurons in the SCN. Our findings provide notable insights into circadian regulation of sleep/wakefulness in mammals.
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影响因子：
3.7
作者：
Ohkura M;Sasaki T;Sadakari J;Gengyo-Ando K;Kagawa-Nagamura Y;Kobayashi C;Ikegaya Y;Nakai J
通讯作者：
Nakai J
DOI：
10.1023/b:visi.0000029664.99615.94
发表时间：
2004-11-01
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作者：
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作者：
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通讯作者：
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DOI：
10.1152/ajpregu.00176.2015
发表时间：
2015-11-01
影响因子：
2.8
作者：
Gvilia, Irma;Suntsova, Natalia;Szymusiak, Ronald
通讯作者：
Szymusiak, Ronald