NEW HUMAN ORAL SQUAMOUS CARCINOMA CELL-LINE AND ITS TUMORIGENIC SUBLINE PRODUCING GRANULOCYTE-COLONY-STIMULATING FACTOR
NEW HUMAN ORAL SQUAMOUS CARCINOMA CELL-LINE AND ITS TUMORIGENIC SUBLINE PRODUCING GRANULOCYTE-COLONY-STIMULATING FACTOR
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DOI：
10.1111/j.1349-7006.1994.tb02938.x
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发表时间：
1994-12-01
期刊：
影响因子：
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通讯作者：
SAKAI, H
中科院分区：
文献类型：
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作者：
MATSUO, K;ISHIBASHI, Y;SAKAI, H
从口腔鳞状细胞癌的转移淋巴结中建立了一种新的人类癌细胞系 MISK81-5。免疫细胞化学和超微结构观察揭示了细胞系明显的上皮起源。染色体分析揭示了具有数值和结构异常的超三倍体核型。仅当与Matrigel共注射时，MISK81-5细胞才能在受体BALB/c无胸腺小鼠的皮下组织中形成肿瘤块。干细胞测定显示 MISK81-5 的条件培养基 (CM) 含有粒细胞集落刺激因子 (G-CSF) 或白细胞介素 6 活性。 ELISA 定量显示，MISK81-5 的 CM 中的 G-CSF 浓度高于其他鳞状细胞系和胃癌细胞系的 CM。源自作为具有较高致瘤性的细胞系亚群MISK81-5的sMISK也显示出与MISK81-5相似的造血刺激活性。 MISK81-5 细胞系及其亚群 sMISK 的这些特征将有助于研究口腔鳞状细胞癌的生物学行为及其与造血刺激因子的关系。
A new human carcinoma cell line, MISK81-5, was established from a metastatic lymph node of oral squamous cell carcinoma. Immunocytochemical and ultrastructural observations revealed an obvious epithelial origin of the cell line. Chromosome analysis revealed a hypertriploid karyotype with numerical and structural anomalies. MISK81-5 cells could form a tumor mass in the subcutaneous tissue of recipient BALB/c athymic mice only when coinjected with Matrigel. A stem cell assay revealed that conditioned medium (CM) of MISK81-5 contained granulocyte colony-stimulating factor (G-CSF) or interleukin-6 activity. Quantitation by ELISA disclosed a higher concentration of G-CSF in the CM of MISK81-5 than in the CM of other squamous and gastric carcinoma cell lines. The sMISK, that was derived from MISK81-5 as a subpopulation of the cell line having higher tumorigenicity, also showed a similar hematopoietic stimulating activity to that of MISK81-5. These characteristics of the MISK81-5 cell line and its subpopulation, sMISK will be useful for studying the biological behavior of oral squamous cell carcinomas and its relation to hematopoietic stimulating factors.