Development of a High-Throughput and Miniaturized Cytokinesis-Block Micronucleus Assay for Use as a Biological Dosimetry Population Triage Tool.
Development of a High-Throughput and Miniaturized Cytokinesis-Block Micronucleus Assay for Use as a Biological Dosimetry Population Triage Tool.
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DOI：
10.1667/rr13991.1
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发表时间：
2015-08
影响因子：
3.4
通讯作者：
Brenner DJ
中科院分区：
文献类型：
--
作者：
Lue SW;Repin M;Mahnke R;Brenner DJ
生物剂量测定是及时评估辐射照射的一个重要工具。对于涉及电离辐射照射的大规模伤亡事件，迅速提供剂量信息以供医疗救治至关重要。成熟的微核阻断试验（CBMN）是一种经验证的生物剂量测定方法。然而，CBMN测定法需要加速样品处理才能成为合适的群体分诊工具。我们在这里报告的发展，一个高通量和小型化版本的CMBN测定加速样品处理。
Biodosimetry is an essential tool for providing timely assessments of radiation exposure. For a large mass-casualty event involving exposure to ionizing radiation, it is of utmost importance to rapidly provide dose information for medical treatment. The well-established cytokinesis-block micronucleus (CBMN) assay is a validated method for biodosimetry. However, the need for an accelerated sample processing is required for the CBMN assay to be a suitable population triage tool. We report here on the development of a high-throughput and miniaturized version of the CMBN assay for accelerated sample processing.