CHEMI-LUMINESCENT ASSAY OF LIPID HYDROPEROXIDES
CHEMI-LUMINESCENT ASSAY OF LIPID HYDROPEROXIDES
复制标题
DOI：
10.1002/bio.1170020302
复制
发表时间：
1988-07-01
期刊：
影响因子：
--
通讯作者：
DEPAULET, AC
中科院分区：
文献类型：
--
作者：
BELGHMI, K;NICOLAS, JC;DEPAULET, AC
在含有少量脂质过氧化氢的溶液中加入鲁米诺和催化剂，如过氧化氢酶或血红素修复基团，可产生一道化学发光，其强度是过氧化氢浓度的函数。脂质氢过氧化氢测定的各种方法已经被描述，我们已经研究了提高它们的灵敏度和特异性的条件。由于血浆中存在的化合物干扰光发射，血浆脂质氢过氧化氢测定需要萃取。此外，测定的灵敏度是由于介质中存在过氧化氢，这导致了较高的背景值。过氧化氢酶不作用于脂质过氧化氢，可用于从反应介质中消除过氧化氢。测定需要一个空白试管，在空白试管中，通过将样品与高铁血红素和抗坏血酸孵育来销毁过氧化氢。然后，将由脂质氢氧化物的存在引起的分析管的化学发光增加与内标物的值进行比较。
The addition of luminol plus a catalyst such as peroxidase or a heme prosthetic group to a solution containing a small quantity of lipid hydroperoxides results in a flash of chemiluminescence, the intensity of which is a function of the hydroperoxide concentrations. Various protocols for lipid hydroperoxide assays have been described and we have studied conditions to increase their sensitivity and specificity.Plasma lipid hydroperoxide determinations require an extraction, since compounds present in plasma interfere with light emission. Moreover, the sensitivity of the assay is by the presence of hydrogen peroxide in the medium, which causes high background values. Catalase does not act on lipid hydroperoxides and can be used to eliminate hydrogen peroxide from the reaction medium. The determination requires a blank tube in which hydroperoxides are destroyed by incubating the sample with haematin plus ascorbate. The increase in the chemiluminescence of the assay tube caused by the presence of lipid hydroperoxides is then compared to the value obtained for an internal standard.