Interactions of HSP22 (HSPB8) with HSP20, αB-crystallin, and HSPB3
Interactions of HSP22 (HSPB8) with HSP20, αB-crystallin, and HSPB3
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DOI：
10.1016/j.bbrc.2005.09.148
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发表时间：
2005-11-25
影响因子：
3.1
通讯作者：
Welsh, MJ
中科院分区：
文献类型：
--
作者：
Fontaine, JM;Sun, XK;Welsh, MJ
在哺乳动物的10种小热休克蛋白中，有7种在肌肉中表达，占总蛋白的3%或更多。SHSP相互作用，这些相互作用被认为对它们的功能很重要。在表达多个sHSP的细胞类型中，了解哪些sHSP相互作用是很有意义的。我们以前已经证明HSP22与自身以及与HSP27、MKBP和cvHSP相互作用。使用酵母双杂交分析和Forster共振能量转移显微镜，我们现在证明HSP22也可以与SHSP家族的另外两个成员相互作用，αB-晶体蛋白和HSP20。我们还发现，HSP22存在于灵长类动物心肌中含有包括α-B-晶体蛋白和HSP20在内的高分子量复合体的高效液相色谱组分中。我们的结果表明，在表达多个sHSP的细胞类型中，可能会形成由不同比例的不同sHSP组成的各种寡聚体。(C)2005 Elsevier Inc.保留所有权利。
Seven of the 10 mammalian small heat shock proteins (sHSP) are expressed in muscle where they constitute 3% or more of total protein. sHSPs interact with one another, and these interactions are believed to be important for their functions. In cell types expressing multiple sHSPs, it is of interest to know which sHSPs interact with one another. We have previously shown that HSP22 interacts with itself as well as with HSP27, MKBP, and cvHSP. Using yeast two-hybrid assays and Forster resonance energy transfer microscopy, we now show that HSP22 also can interact with two additional members of the sHSP family, alpha B-crystallin and HSP20. We also show that HSP22 is found in HPLC fractions of primate cardiac muscle containing high molecular weight complexes that include alpha B-crystallin and HSP20. Our results suggest that a variety of oligomers composed of different proportions of different sHSPs may form in cell types expressing multiple sHSPs. (c) 2005 Elsevier Inc. All rights reserved.