Novel neurotrophic effects of sphingosylphosphorylcholine in cerebellar granule neurons and in PC12 cells
Novel neurotrophic effects of sphingosylphosphorylcholine in cerebellar granule neurons and in PC12 cells
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DOI：
10.1016/j.bbrc.2007.09.121
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发表时间：
2007-12-07
影响因子：
3.1
通讯作者：
Arioka, Manabu
中科院分区：
文献类型：
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作者：
Konno, Naoko;Nakamura, Ayako;Arioka, Manabu
鞘氨醇磷酸胆碱（SPC）是一种天然存在的鞘脂衍生物，参与各种生物过程。在这里，我们表明，SPC显示小脑颗粒神经元（CGNs）和PC12细胞的神经营养作用。当CGN在非去极化条件下培养时，它们表现出典型的凋亡表型的浓缩和碎裂的核。加入到培养基中的SPC拯救了经历凋亡的细胞。SPC的抗凋亡作用依赖于细胞外Ca 2+的存在，表明SPC处理后发生Ca 2+内流。在PC12细胞中，SPC表现出神经生长因子样的神经突发生作用，该作用对Ca 2+通道阻断剂的存在和培养基中Ca 2+的撤出敏感。这些结果表明，SPC在神经系统中发挥新的神经营养作用。(C)2007年爱思唯尔公司All rights reserved.
Sphingosylphosphorylcholine (SPC) is a choline-containing naturally occurring derivative of sphingolipid involved in various biological processes. Here we show that SPC displays neurotrophic effects in cerebellar granule neurons (CGNs) and in PC12 cells. When CGNs were cultured under non-depolarizing condition, they exhibited condensed and fragmented nuclei typical of apoptotic phenotype. SPC added to the culture medium rescued cells from undergoing apoptosis. The anti-apoptotic effect of SPC was dependent on the presence of extracellular Ca2+, suggesting that Ca2+ influx occurs upon SPC treatment. In PC12 cells, SPC displayed nerve growth factor-like neuritogenic effect which was sensitive to the presence of Ca2+ channel blocker and Ca2+ withdrawal from the medium. These results suggest that SPC plays novel neurotrophic effects in the nervous system. (C) 2007 Elsevier Inc. All rights reserved.