Investigation into the causes of canine infectious respiratory disease: antibody responses to canine respiratory coronavirus and canine herpesvirus in two kennelled dog populations
Investigation into the causes of canine infectious respiratory disease: antibody responses to canine respiratory coronavirus and canine herpesvirus in two kennelled dog populations
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DOI：
10.1007/s00705-005-0533-x
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发表时间：
2005-08-01
影响因子：
2.7
通讯作者：
Brownlie, J
中科院分区：
文献类型：
--
作者：
Erles, K;Brownlie, J
对两个工作犬训练中心进行了为期一年的监测，以确定病毒和病毒抗体的存在及其与犬传染性呼吸道疾病（CIRD）的关系。在犬进入犬舍时以及此后定期采集犬的扁桃体拭子和血清。在CIRD爆发期间收集了额外的样本。通过病毒培养和PCR检测犬副流感病毒、犬腺病毒、犬疱疹病毒（CHV）和犬呼吸道冠状病毒（CRCoV）。此外，还确定了CHV和CRCoV抗体的流行率。在本研究期间，CIRD主要在所研究的两个犬舍之一中报告。在该犬舍中，对CRCoV的抗体反应表明该病毒的季节性发生，这与两次呼吸道疾病的爆发相吻合。全年都检测到CHV抗体应答。在另一个犬舍中，报告了很少的CIRD病例，在进入时检测到CRCoV抗体的高流行率，但仅零星地检测到CRCoV或CHV的血清转化。通过PCR，在一个犬舍中发现三只狗对CRCoV呈阳性，而在两个犬舍中对其他病毒的所有PCR检测均为阴性。病毒培养未能在任一犬舍中检测到任何病毒。
Two training centres for working dogs were monitored for one year to determine the presence of viruses and viral antibodies and their association with canine infectious respiratory disease (CIRD). Tonsillar swabs and serum were obtained from dogs on entry into the kennels and in regular intervals thereafter. Additional samples were collected during outbreaks of CIRD. The swabs were examined by virus culture and PCR for canine parainfluenza virus, canine adenovirus, canine herpesvirus (CHV) and canine respiratory coronavirus (CRCoV). Furthermore the prevalence of antibodies to CHV and CRCoV was determined. During this study CIRD was reported mainly in one of the two kennels investigated. In that kennel antibody responses to CRCoV indicated a seasonal occurrence of the virus, which coincided with two outbreaks of respiratory disease. CHV antibody responses were detected throughout the year. In the other kennel, which reported few cases of CIRD a high prevalence of antibodies to CRCoV was detected on entry but only sporadic seroconversions to CRCoV or CHV. By PCR three dogs were found positive for CRCoV in one kennel whereas all PCR tests for other viruses were negative for both kennels. Virus culture failed to detect any viruses in either kennel.