Proteomic Profiling Analysis of Male Infertility in Spodoptera Litura Larvae Challenged with Azadirachtin and its Potential-Regulated Pathways in the Following Stages
Proteomic Profiling Analysis of Male Infertility in Spodoptera Litura Larvae Challenged with Azadirachtin and its Potential-Regulated Pathways in the Following Stages
复制标题
印楝素攻击斜纹夜蛾幼虫雄性不育的蛋白质组学分析及其后续阶段的潜在调控途径
DOI：
10.1002/pmic.201800192
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发表时间：
2018
期刊：
影响因子：
3.4
通讯作者：
Yi Xin
中科院分区：
文献类型：
--
作者：
Sun Ranran;Cui Gaofeng;Chen Yaoyao;Shu Benshui;Zhong Guohua;Yi Xin
Biopesticides are considered as an alternative to synthetic pesticide with a focus on increasing agricultural productivity as well as maintaining the ecosystem. Prior to application, its potential mechanism should be clearly addressed. Here, the effects of azadirachtin on the reproductive behavior in maleSpodoptera litura(Fabricius) are determined. To further explore its molecular mechanism, an iTRAQ (isobaric tags for relative and absolute quantitation) based approach is applied to identify the differentially expressed proteins regulated by azadirachtin at two developmental stages. The results demonstrate that many proteins in the pathway of focal adhesion are regulated to exert influences in detachment of cell attachment, the loss of cell–cell interactions and inducing apoptosis at pupal stage, and many proteins in adenosine monophosphate‐activated protein kinase pathway are also changed at the adult stage after azadirachtin‐treatment as larvae. Moreover, based on their important roles, it is suggested that some proteins, such as ACTB‐G1, ste20‐related adaptor protein alpha, and regulatory‐associated protein of mTOR (mTORC1) could serve as potential target proteins of azadirachtin to induce male infertility. The results of this study could provide evidence to illuminate the mechanism of male infertility induced by azadirachtin and potential targets for the development of environmentally friendly pesticides.
