A CELLULAR ONCOGENE IS TRANSLOCATED TO THE PHILADELPHIA-CHROMOSOME IN CHRONIC MYELOCYTIC-LEUKEMIA
A CELLULAR ONCOGENE IS TRANSLOCATED TO THE PHILADELPHIA-CHROMOSOME IN CHRONIC MYELOCYTIC-LEUKEMIA
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DOI：
10.1038/300765a0
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发表时间：
1982-01-01
期刊：
影响因子：
64.8
通讯作者：
STEPHENSON, JR
中科院分区：
文献类型：
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作者：
DEKLEIN, A;VANKESSEL, AG;STEPHENSON, JR
致癌逆转录病毒的转化基因与一组进化上保守的细胞癌基因同源。最近发现Abelson鼠白血病病毒（A-MuLV）转化序列的人类细胞同源物（c-abl）位于9号染色体上。该染色体的长臂与22号染色体的特异性易位有关，即费城易位（Ph 1），t（9;22）（q34，q11），发生在慢性粒细胞白血病（CML）3-5患者中。在这里，我们调查是否c-ablgene是包括在这个易位。使用c-abl和v-abl杂交探针对体细胞杂种进行印迹，当22 q −（费城染色体）而不是易位的9 q+衍生物存在于细胞杂种中时，发现阳性杂交。由此我们得出结论，在CML中，c-ab序列从9号染色体易位到22号染色体q-。这一发现直接证明了两个染色体之间的相互交换，并表明c-ab基因在CML的产生中起作用。
The transforming genes of oncogenic retroviruses are homologous to a group of evolutionary conserved cellularoncgenes1. The human cellular homologue (c-abl) of the transforming sequence of Abelson murine leukaemia virus (A-MuLV) was recently shown2to be located on chromosome 9. The long arm of this chromosome is involved in a specific translocation with chromosome 22, the Philadelphia translocation (Ph1), t(9;22) (q34, q11), which occurs in patients with chronic myelocytic leukaemia (CML)3–5. Here we investigate whether the c-ablgene is included in this translocation. Using c-abland v-ablhybridization probes on blots of somatic cell hybrids, positive hybridization is found when the 22q−(the Philadelphia chromosome), and not the 9q+derivative of the translocation, is present in the cell hybrids. From this we conclude that in CML, c-ablsequences are translocated from chromosome 9 to chromosome 22q−. This finding is a direct demonstration of a reciprocal exchange between the two chromosomes6and suggests a role for the c-ablgene in the generation of CML.