Elevated levels of interleukin-18 and tumor necrosis factor-α in serum of patients with type 2 diabetes mellitus:: Relationship with diabetic nephropathy
Elevated levels of interleukin-18 and tumor necrosis factor-α in serum of patients with type 2 diabetes mellitus:: Relationship with diabetic nephropathy
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DOI：
10.1053/meta.2003.50096
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发表时间：
2003-05-01
影响因子：
9.8
通讯作者：
Hada, T
中科院分区：
文献类型：
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作者：
Moriwaki, Y;Yamamoto, T;Hada, T
为了比较血清中白细胞介素（IL）-18、肿瘤坏死因子-α（TNF-α）和IL-6的水平，我们研究了151例伴有不同程度肾病的2型糖尿病患者以及80名健康志愿者。使用酶联免疫吸附测定（ELISA），用相应的小鼠单克隆抗体测量血清中的IL-18、TNF-α和IL-6。在患者和对照组之间观察到血清IL-18和TNF-α水平的显著差异（IL-18，278.0 +/- 11.9 pg/mL vs 172.8 +/- 7.7 pg/mL，P < .0001; TNF-α，2.41 +/- 0.18 pg/mL v0.46 +/- 0.18 pg/mL，P <0.0001），而IL-6在两组之间无差异（0.73 +/- 0.10 pg/mL v 0.65 +/- 0.08 pg/mL，差异不显著[NS]），尽管肾病患者的水平较高。此外，随着尿白蛋白排泄的发展，糖尿病患者的IL-18水平升高，在微量白蛋白尿患者中发现最高水平（300 mug/mg肌酐，350.0 +/- 16.0 pg/mL）。同样，与无蛋白尿的患者相比，有微量白蛋白尿或临床白蛋白尿的糖尿病患者TNF-α和IL-6显著升高（TNF-α，3.20 +/- 0.41 pg/mL vs 1.94 +/- 0.18 pg/mL; IL-6，1.64 +/- 1.11 pg/mL vs 0.51 +/- 0.05 pg/mL，P <0.05）。这些结果表明，血清IL-18，TNF-α和IL-6水平可能在糖尿病肾病中具有一定的病因作用。(C)2003年爱思唯尔公司All rights reserved.
To compare levels of interleukin (IL)-18, tumor necrosis factor-alpha (TNF-alpha), and IL-6 in serum, we studied 151 type 2 diabetes mellitus patients with various degrees of nephropathy, as well as 80 healthy volunteers. IL-18, TNF-alpha, and IL-6 in serum were measured using an enzyme-linked immunosorbent assay (ELISA) with the respective mouse monoclonal antibodies. Significant differences in serum levels of IL-18 and TNF-alpha were observed between the patients and control subjects (IL-18, 278.0 +/- 11.9 pg/mL v 172.8 +/- 7.7 pg/mL, P < .0001; TNF-alpha, 2.41 +/- 0.18 pg/mL v 0.46 +/- 0.18 pg/mL, P < .0001), whereas that of IL-6 was not different between the two groups (0.73 +/- 0.10 pg/mL v 0.65 +/- 0.08 pg/mL, difference not significant [NS]), although patients with nephropathy showed higher levels. In addition, IL-18 levels were increased in diabetic patients with the development of urinary albumin excretion, with the highest found in those with microalbuminuria (300 mug/mg creatinine, 350.0 +/- 16.0 pg/mL). Similarly, TNF-alpha and IL-6 in diabetic patients with microalbuminuria or clinical albuminuria were significantly increased as compared with those without albuminuria (TNF-alpha, 3.20 +/- 0.41 pg/mL v 1.94 +/- 0.18 pg/mL; IL-6,1.64 +/- 1.11 pg/mL v 0.51 +/- 0.05 pg/mL, P < .05, respectively). These results suggest that serum levels of IL-18, TNF-alpha, and IL-6 may have some etiopathogenic roles in diabetic nephropathy. (C) 2003 Elsevier Inc. All rights reserved.