STRUCTURE OF THE PLASMODIUM-KNOWLESI GENE CODING FOR THE CIRCUMSPOROZOITE PROTEIN
STRUCTURE OF THE PLASMODIUM-KNOWLESI GENE CODING FOR THE CIRCUMSPOROZOITE PROTEIN
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DOI：
10.1016/0092-8674(83)90538-x
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发表时间：
1983-01-01
期刊：
影响因子：
64.5
通讯作者：
GODSON, GN
中科院分区：
文献类型：
--
作者：
OZAKI, LS;SVEC, P;GODSON, GN
编码诺氏疟原虫子孢子表面抗原（CS蛋白）的基因是未分裂的，仅以1个拷贝存在于基因组中。从结构基因的DNA序列分析推断，CS蛋白具有不寻常的结构，其中中心40%的多肽链作为12个串联重复的氨基酸肽单元存在，两侧是高电荷氨基酸区域。该蛋白质具有氨基端疏水氨基酸信号序列和疏水羧基端锚序列。该基因的编码序列的AT含量为53%，而5”和3”侧翼序列的AT含量为70%，并且完全包含在11 kb [千碱基] Eco RI基因组DNA片段内。该基因组片段在大肠杆菌中表达CS蛋白，表明寄生虫启动子和核糖体结合位点信号在大肠杆菌中可以被识别。杆菌
The gene that codes for the surface antigen of P. knowlesi sporozoites (CS protein) is unsplit and present in the genome in only 1 copy. The CS protein, as deduced from DNA sequence analysis of the structural gene, has an unusual structure with the central 40% of the polypeptide chain present as 12 tandemly repeated amino acid peptide units flanked by regions of highly charged amino acids. The protein has an amino-terminal hydrophobic amino acid signal sequence and a hydrophobic carboxy-terminal anchor sequence. The coding sequence of the gene has an AT content of 53%, compared with 70% AT in the 5'' and 3'' flanking sequences, and is contained entirely within an 11 kb [kilobase] Eco RI genomic DNA fragment. This genomic fragment expresses the CS protein in Escherichia coli, indicating that the parasite promoter and ribosome binding site signals can be recognized in E. coli.