Effects of an Early Handling-Like Procedure and Individual Housing on Anxiety-Like Behavior in Adult C57BL/6J and DBA/2J Mice
Effects of an Early Handling-Like Procedure and Individual Housing on Anxiety-Like Behavior in Adult C57BL/6J and DBA/2J Mice
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DOI：
10.1371/journal.pone.0019058
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发表时间：
2011-04-21
期刊：
影响因子：
3.7
通讯作者：
Cook, Melloni N.
中科院分区：
文献类型：
--
作者：
Flanigan, Timothy J.;Cook, Melloni N.
操纵养育条件已被用来研究早期经验对成年人行为的影响，结果各不相同。有证据表明，出生后第15-21天（PND）是对小鼠焦虑样行为的环境影响特别敏感的时间。为了验证这一点，我们对C57 BL/6 J和DBA/2 J小鼠进行了早期处理样程序。从PND 12-20开始，每天将幼仔与母鼠分开30分钟或接受标准护理。PND 21时，幼仔断奶，单独或分组饲养。在PND 60时，在高架零迷宫上检查焦虑样行为。虽然单独圈养的动物需要更长的时间才能进入迷宫的开放象限，但它们在开放象限中的时间比分组圈养的动物多。此外，我们观察到C57 BL/6 J小鼠的焦虑样行为减少的趋势，而经历处理样程序的DBA/2 J小鼠则没有。
Manipulations of rearing conditions have been used to examine the effects of early experience on adult behavior with varying results. Evidence suggests that postnatal days (PND) 15-21 are a time of particular susceptibility to environmental influences on anxiety-like behavior in mice. To examine this, we subjected C57BL/6J and DBA/2J mice to an early handling-like procedure. Pups were separated from dams from PND 12-20 for 30 minutes daily or received standard care. On PND 21, pups were weaned and either individually- or group-housed. On PND 60, anxiety-like behavior was examined on the elevated zero-maze. Although individually- housed animals took longer to enter an open quadrant of the maze, they spent more time in the open than group-housed animals. Additionally, we observed a trend of reduced anxiety-like behavior in C57BL/6J, but not DBA/2J mice that underwent the handling-like procedure.