Identification and analysis of the metacaspase gene family in tomato
Identification and analysis of the metacaspase gene family in tomato
复制标题
番茄metacaspase基因家族的鉴定与分析
DOI：
10.1016/j.bbrc.2016.09.103
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发表时间：
2016-10-21
影响因子：
3.1
通讯作者：
Wei, Yongxuan
中科院分区：
文献类型：
--
作者：
Liu, Hui;Liu, Jian;Wei, Yongxuan
在发育调节和环境诱导的植物细胞程序性死亡中，Metacaspase起着关键作用。本研究系统地鉴定和分析了番茄(Solanum Lycopsicum)的Metacaspase基因家族。结果表明，番茄具有8个偏置酶基因(SlMC1-8)，分别位于第1、3、5、9、10号染色体上。SLMC1-6属于I型偏置酶，具有5个外显子/4个内含子结构。SIMC7和SIMC8分别含有2个和3个外显子。表达分析表明，SLMCs在番茄不同组织中的表达模式不同。顺式调控元件预测表明，在SLMCs启动子区域存在许多与激素和胁迫相关的顺式调节元件。实时定量聚合酶链式反应分析进一步证明，大多数SLMC受干旱、寒冷、盐、甲基紫精和乙烯利处理的调控。本研究揭示了番茄SLMC基因的特点，为进一步研究这些基因的功能奠定了基础。(C)2016 Elsevier Inc.保留所有权利。
Metacaspases play critical roles in developmentally regulated and environmentally induced programmed cell death in plants. In this study, we systematically identified and analyzed metacaspase gene family in tomato (Solanum lycopersicum). The results illustrated that tomato possesses eight metacaspase genes (SlMC1-8) located on chromosomes 1, 3, 5, 9, and 10. SlMC1-6 belonged to type I metacaspases and had 5 exon/4 intron structures. SIMC7 and 8 were type II metacaspases and had 2 and 3 exons, respectively. Expression analysis revealed distinct expression patterns of SlMCs in various tomato tissues. Cis-regulatory element prediction showed that there were many hormone- and stress-related cis-regulatory elements in SlMCs promoter regions. Quantitative real-time PCR analysis further demonstrated that most of the SlMCs were regulated by drought, cold, salt, methyl viologen, and ethephon treatments. This study provides insights into the characteristics of SlMC genes and laid the foundation for further functional analysis of these genes in tomato. (C) 2016 Elsevier Inc. All rights reserved.