Tissue-specific expression pattern of estrogen receptors (ER):: Quantification of ERα and ERβ mRNA with real-time RT-PCR
Tissue-specific expression pattern of estrogen receptors (ER):: Quantification of ERα and ERβ mRNA with real-time RT-PCR
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DOI：
10.1034/j.1600-0463.2001.090503.x
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发表时间：
2001-05-01
期刊：
影响因子：
2.8
通讯作者：
Meyer, HHD
中科院分区：
文献类型：
--
作者：
Pfaffl, MW;Lange, IG;Meyer, HHD
我们已经研究了组织特异性mRNA表达的ER α和ERP在各种牛组织中使用实时RT-PCR。本研究的目的是评估偏离组织敏感性和雌激素活性制剂RALGRO对组织特异性表达和调节的两种ER亚型的影响。RALCRO含有Zeranol（α-玉米赤霉醇），一种真菌毒素玉米赤霉烯酮的衍生物，显示出强烈的雌激素和合成代谢作用，并表现出雌激素过多的所有症状，特别是生殖和发育障碍。8头小母牛在8周内接受Zeranol多次给药（0 x、1 x、3 x、10 x）。血浆玉米赤霉醇浓度，酶免疫测定，多次治疗的小母牛升高。为了在低丰度组织中定量ER α和ERP转录本，开发了灵敏可靠的实时RT-PCR定量方法，并在LightCycler上进行了验证。表达结果表明，在所有15个研究组织中存在两种ER亚型。所有组织都表现出特定的ER α和ERP表达模式和调控。随着玉米赤霉醇浓度的增加，空肠ER α mRNA表达显著下调（p
We have examined the tissue-specific mRNA expression of ERa and ERP in various bovine tissues using real-time RT-PCR. The goal of this study was to evaluate the deviating tissue sensitivities and the influence of the estrogenic active preparation RALGRO on the tissue-specific expression and regulation of both ER subtypes. RALCRO contains Zeranol (alpha -Zearalanol), a derivative of the mycotoxin Zearalenon, shows strong estrogenic and anabolic effects, and exhibits all symptoms of hyperestrogenism, in particular reproductive and developmental disorders. Eight heifers were treated over 8 weeks with multiple-dose implantations (0x, 1x, 3x, 10x) of Zeranol. Plasma Zeranol concentration, measured by enzyme immunoassay, of multiple treated heifers was elevated. To quantify ERa and ERP transcripts also in low-abundant tissues, sensitive and reliable real-time RT-PCR quantification methods were developed and validated on the LightCycler. Expression results indicate the existence of both ER subtypes in all 15 investigated tissues. All tissues exhibited a specific ERa and ERP expression pattern and regulation. With increasing Zeranol concentrations, a significant downregulation of ERa mRNA expression could be observed in jejunum (p