SOCIAL-INTERACTION INCREASES 5-HT RELEASE AND CAMP EFFLUX IN THE RAT VENTRAL HIPPOCAMPUS IN-VIVO
SOCIAL-INTERACTION INCREASES 5-HT RELEASE AND CAMP EFFLUX IN THE RAT VENTRAL HIPPOCAMPUS IN-VIVO
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DOI：
10.1097/00008877-199406000-00007
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发表时间：
1994-06-01
影响因子：
1.6
通讯作者：
MARSDEN, CA
中科院分区：
文献类型：
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作者：
CADOGAN, AK;KENDALL, DA;MARSDEN, CA
本研究的目的是结合联合收割机在大鼠体内微透析与行为的社会互动测试，以调查的变化，5-HT释放和环磷酸腺苷（cAMP）流出在腹侧海马的同时测量的行为。暴露的大鼠与一个不熟悉的合作伙伴在一个明亮的，不熟悉的竞技场的社会互动10分钟的时间导致在腹侧海马细胞外5-HT和细胞外cAMP的增加。在社会互动测试前30分钟用地西泮（1 mg/kg i.p）预处理显著抑制细胞外5-HT和cAMP的增加，同时显著增加了一对大鼠在10分钟内进行积极社会接触的时间。在30分钟前的社会互动测试，地西泮降低基础水平的5-HT，但没有影响基础流出的cAMP。用选择性5-HT 1A拮抗剂WAY 100135（5 mg/kg s.c.）预处理，30分钟前的社会互动测试，显着加强增加细胞外5-HT在生理盐水处理的大鼠在社会互动测试过程中观察到。相比之下，WAY 100135预处理显著减弱了在社交互动测试期间在盐水处理的大鼠中观察到的细胞外cAMP的增加，但对成对大鼠之间的主动社交接触所花费的时间没有影响。结果表明，社会互动的结果在激活突触后5-HT受体（5-HT 1A或5-HT 6/5-HT 7）耦合腺苷酸环化酶，但该受体是不负责的厌恶诱导的行为。此外，在5-HT神经元激活的条件下，而不是在基础条件下，5-HT 1A体树突自身受体的拮抗作用增强5-HT的释放。
The aim of the present study was to combine in vivo microdialysis in the rat with behaviour in the social interaction test in order to investigate changes in both 5-HT release and cyclic AMP (cAMP) efflux in the ventral hippocampus with simultaneous measurement of behaviour. Exposure of the rat to a 10 min period of social interaction with an unfamiliar partner in a brightly lit, unfamiliar arena resulted in an increase in extracellular 5-HT and extracellular cAMP in the ventral hippocampus. Pretreatment with diazepam (1 mg/kg i.p,) 30 min prior to the social interaction test significantly inhibited the increases in both extracellular 5-HT and cAMP while significantly increasing the amount of time the pair of rats spent in active social contact over the 10 min period. During the 30 min prior to the social interaction test diazepam reduced basal levels of 5-HT, but had no effect on the basal efflux of cAMP. Pretreatment with a selective 5-HT1A antagonist, WAY 100135 (5 mg/kg s.c.), 30 min prior to the social interaction test, significantly potentiated the increase in extracellular 5-HT observed in saline-treated rats during the social interaction test. In contrast, WAY 100135 pretreatment significantly attenuated the increase in extracellular cAMP observed in saline-treated rats during the social interaction test but had no effect on the time spent in active social contact between pairs of rats. The results suggest that social interaction results in activation of a post-synaptic 5-HT receptor (5-HT1A or 5-HT6/5-HT7) coupled to adenylate cyclase but that this receptor is not responsible for the aversion-induced behaviour. Furthermore antagonism of the 5-HT1A somatodendritic autoreceptor under conditions of 5-HT neuronal activation, but not under basal conditions, potentiates 5-HT release.