A Novel Alkali Extractable Polysaccharide from Plantago asiatic L. Seeds and Its Radical-Scavenging and Bile Acid-Binding Activities
A Novel Alkali Extractable Polysaccharide from Plantago asiatic L. Seeds and Its Radical-Scavenging and Bile Acid-Binding Activities
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一种新型车前子碱可提取多糖及其自由基清除和胆汁酸结合活性
DOI：
10.1021/jf505909k
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发表时间：
2015-01-21
影响因子：
6.1
通讯作者：
Yu, Liangli (Lucy)
中科院分区：
文献类型：
--
作者：
Gong, Lu;Zhang, Hua;Yu, Liangli (Lucy)
从车前中分离得到一种新的酸性多糖（PLP）。种子通过热碱提取和使用DEAE纤维素和Sephacryl S-400柱的色谱纯化。PLP的分子量为1.15 × 10（6）Da，单糖组成为木糖（Xyl）、阿拉伯糖（Ara）、葡萄糖醛酸（GlcA）和半乳糖（Gal），摩尔比为18.8：7.2：6.1：1。甲基化分析、FT-IR、1D和2D NMR的结果表明PLP是一种高度支化的杂木聚糖，其主链为β-1，4-连接的Xylp，每8个单糖残基在O-3位连接3个a-GlcAp-（1 -> 3）-Araf，在O-2位连接1个a-T-连接的GlcAp和1个a-Araf-（1 -> 5）-Araf。PLP在体外表现出对羟基、过氧阴离子和DPPH自由基的清除能力，并表现出对胆酸和鹅去氧胆酸的显著结合能力，表明其可能的降胆固醇活性。结果表明，PLP在功能食品和营养保健品中具有潜在的用途。
A new acidic polysaccharide (PLP) was isolated and characterized from Plantago asiatic L. seeds by hot alkali extraction and chromatographic purification using DEAE cellulose and Sephacryl S-400 columns. PLP has a molecular weight of 1.15 x 10(6) Da, and a monosaccharide composition of xylose (Xyl), arabinose (Ara), glucuronic acid (GlcA), and galactose (Gal) in a molar ratio of 18.8:7.2:6.1:1. The results of methylation analysis, FT-IR, and 1D and 2D NMR indicated that PLP was a highly branched heteroxylan of beta-1,4-linked Xylp backbone with three a-GlcAp-(1 -> 3)-Araf attached to the O-3 position and one a-T-linked-GlcAp and one alpha-Araf-(1 -> 5)-Araf attached to the O-2 position every eight monosaccharide residues. PLP exhibited scavenging abilities against hydroxyl, peroxyl anion, and DPPH radicals in vitro and showed significant binding capacities against cholic and chenodeoxycholic acids, suggesting its possible cholesterol-lowering activity. The results demonstrated the potential use of PLP in functional foods and nutraceuticals.