THE MECHANISMS WHICH PRODUCE VACUOLATED AND DEGRANULATED EOSINOPHILS
THE MECHANISMS WHICH PRODUCE VACUOLATED AND DEGRANULATED EOSINOPHILS
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DOI：
10.1111/j.1365-2141.1981.tb07218.x
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发表时间：
1981-01-01
影响因子：
6.5
通讯作者：
SPRY, CJF
中科院分区：
文献类型：
--
作者：
TAI, PC;SPRY, CJF
由于嗜酸性粒细胞增多症患者的血液嗜酸性粒细胞可能含有折射空泡(空泡化的嗜酸性粒细胞)，而一些细胞只含有少数颗粒(脱颗粒的嗜酸性粒细胞)，呈现超微结构的变化，因此，我们进行了实验，以观察正常人的血液嗜酸性粒细胞是否也会发生类似的形态变化。用眼镜蛇毒因子处理的血清培养正常嗜酸性粒细胞过夜，可引起屈光性空泡和颗粒基质的超微结构改变。10微克/毫升刀豆蛋白A作用2 h后，可形成较大的含有颗粒内容物的核周空泡，且颗粒晶体物质的超微结构变化更为明显。细胞松弛素B抑制刀豆蛋白A的上述作用，但这两种刺激均不能引起胞浆颗粒数量的显著减少，但溶血磷脂和不溶性(但不可溶)免疫复合体产生大的内吞空泡，部分溶解的空泡和整个嗜酸性粒细胞被排出。嗜酸性粒细胞颗粒明显可以通过两种不同的方式受到影响。在1中，颗粒基质改变，导致光镜下出现小的屈光性空泡。在第二阶段，将晶体材料分散到基质中。这些明显不同的步骤先于颗粒内容物释放到大的细胞质空间或细胞外。此外，由于患者的嗜酸性粒细胞可能表现出这两种变化中的一种或两种，因此有人认为它们是不同的事件。由于刀豆蛋白A诱导的颗粒增溶不会导致颗粒向外释放，因此提出可能需要额外的刺激来产生嗜酸性粒细胞颗粒内容物的胞吐。进一步的研究可能会显示这些过程是如何联系在一起的，以及它们在嗜酸性粒细胞效应器功能中的相对重要性。
As blood eosinophils in patients with an eosinophilia may contain refractile vacuoles (vacuolated eosinophils), and some cells contain only a few granules (degranulated eosinophils) which show ultrastructural changes, experiments were done to see whether similar morphological alterations could be induced in blood eosinophils from normal people. Overnight culture of normal eosinophils with serum treated with cobra venom factor induced refractile vacuoles and ultrastructural changes in granule matrix material. Concanavalin A, 10 .mu.g/ml for 2 h, led to the formation of larger perinuclear vacuoles containing granule contents, and ultrastructural changes were more marked in granule crystalloid material. Cytochalasin B inhibited these effects of concanavalin A. Neither of these stimuli caused a marked reduction in the number of cytoplasmic granules, but lysolecithin and insoluble (but not soluble) immune complexes produced large endocytic vacuoles into which partially solubilized and whole eosinophil granules were discharged. Eosinophil granules evidently can be affected in 2 distinct ways. In 1, the granule matrix is altered leading to the light microscopy appearances of small refractile vacuoles. In the 2nd, the crystalloid material is dispersed into the matrix. These apparently distinct steps precede the release of the granule contents into large cytoplasmic spaces or to the outside of the cell. Also, as patients'' eosinophils may show one or both of these alterations, it is suggested that they are distinct events. As concanavalin A induced solubilization of granules did not result in release to the outside, it is proposed that an additional stimulus may be required to produce exocytosis of eosinophil granule contents. Further studies may show how these processes are linked and their relative importance in eosinophil effector functions.