MS/MS molecular networking‐guided in‐depth profiling of triterpenoid saponins from the fruit of Eleutherococcus senticosus and their neuroprotectivity evaluation
MS/MS molecular networking‐guided in‐depth profiling of triterpenoid saponins from the fruit of Eleutherococcus senticosus and their neuroprotectivity evaluation
复制标题
MS/MS 分子网络——指导刺五加果实中三萜皂苷的深入分析及其神经保护性评估
DOI：
10.1002/pca.3198
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发表时间：
2022
影响因子：
3.3
通讯作者：
Yue‐Wei Ge
中科院分区：
文献类型：
--
作者：
Yu Zhou;Ying‐Shan Ren;Xi‐Tao Li;Meng‐Ting Cai;Hui‐Lin Li;Wen‐Luan Ding;Yu‐Hang Wu;Hai‐Biao Guo;Zhong‐Hua Tang;Fei Sun;A‐Li Chen;Xiu‐Hong Piao;Shu‐Mei Wang;Yue‐Wei Ge
刺五加是一种天然的保健品资源，被广泛应用于神经系统的滋补。三萜皂苷是ESF的主要成分，我们对ESF粗提物中的TS进行了全面深入的基于MS/MS分子网络（MN）的靶向分析，并研究了其神经保护价值。方法使用MS/MS MN引导策略快速呈现一系列前体离子（PI），作为TS的发现和表征中使用的TS靶向信息。此外，一个准备TS-丰富的部分ESF进行了测定其抑制作用β-淀粉样蛋白诱导的抑制神经突outgrowth.ResultsA共87 TS被发现使用PI跟踪策略，其中28个被表征为潜在的未描述的结构，根据其高分辨率MS值。此外，TS-丰富的馏分可以显着减少β-淀粉样蛋白诱导的神经网络损伤，促进突起和轴突的outgrowth.ConclusionOur研究结果揭示了丰富的TS在ESF，并将加速其在神经退行性疾病的治疗应用。
IntroductionEleutherococcus senticosusfruit (ESF) is a natural health supplement resource that has been extensively applied as a tonic for the nervous system. The structures and neural bioactivities of triterpenoid saponins (TS), which are the major constituents of ESF, have not been comprehensively analyzed thus far.ObjectiveWe conducted a complete in‐depth MS/MS molecular networking (MN)‐based targeted analysis of TS from the crude extract of ESF and investigated its neuroprotective value.MethodsAn MS/MS MN‐guided strategy was used to rapidly present a series of precursor ions (PIs) of TS in a compound cluster as TS‐targeted information used in the discovery and characterization of TS. In addition, a prepared TS‐rich fraction of ESF was assayed for its restraining effects on β‐amyloid‐induced inhibition of neurite outgrowth.ResultsA total of 87 TS were discovered using a PI tracking strategy, 28 of which were characterized as potentially undescribed structures according to their high‐resolution MS values. Furthermore, the TS‐rich fraction can significantly reduce β‐amyloid‐induced damage to neural networks by promoting the outgrowth of neurites and axons.ConclusionOur findings reveal the richness of TS in ESF and will accelerate their application in the treatment of neurodegenerative diseases.